LOCUS       BBa_K079006             1625 bp    DNA     linear       25-Nov-2024
DEFINITION  TetR protein with RBS and GFP reporter protein
ACCESSION   BBa_K079006
VERSION     BBa_K079006.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             22..638
                     /label=tetR
     misc_feature    639..672
                     /label=SsrA
     misc_feature    679..703
                     /label=Help:Barcodes
     CDS             19..678
                     /label=BBa_C0040
     misc_feature    716..719
                     /label=conserved
     RBS             712..723
                     /label=BBa_B0034
     misc_feature    730..1449
                     /label=BBa_E0040
     misc_feature    1444..1482
                     /label=LVA
     misc_feature    1483..1488
                     /label=Double Stop
     misc_feature    730..732
                     /label=Start
     misc_feature    730..1488
                     /label=BBa_J04031
     stem_loop       1508..1551
                     /label=stem_loop
     misc_feature    1497..1576
                     /label=BBa_B0010
     terminator      1497..1576
                     /label=BBa_B0010
     stem_loop       1592..1611
                     /label=T7 TE
     misc_feature    1618..1618
                     /label=stop
     polyA_site      1612..1625
                     /label=polya
     misc_feature    1585..1625
                     /label=BBa_B0012
     terminator      1585..1625
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
       61 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      121 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      181 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      241 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      301 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      361 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      421 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      481 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      541 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
      601 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
      661 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gaaagaggag
      721 aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
      781 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
      841 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
      901 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat
      961 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
     1021 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
     1081 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
     1141 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
     1201 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
     1261 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
     1321 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     1381 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
     1441 aaaaggcctg ctgcaaacga cgaaaactac gctttagtag cttaataata ctagagccag
     1501 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1561 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1621 ttata
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