LOCUS BBa_K079013 4273 bp DNA linear 22-Nov-2024 DEFINITION fs ACCESSION BBa_K079013 VERSION BBa_K079013.1 FEATURES Location/Qualifiers misc_feature 1090..1128 /label=LVA CDS 1..1128 /label=lacI-LVA misc_feature 1..1128 /label=BBa_C0012 misc_feature 1129..1153 /label=Help:Barcodes CDS 1..1128 /label=BBa_C0012 stem_loop 1173..1216 /label=stem_loop misc_feature 1162..1241 /label=BBa_B0010 terminator 1162..1241 /label=BBa_B0010 stem_loop 1257..1276 /label=T7 TE misc_feature 1283..1283 /label=stop polyA_site 1277..1290 /label=polya misc_feature 1250..1290 /label=BBa_B0012 terminator 1250..1290 /label=BBa_B0012 misc_feature 2386..2424 /label=LVA CDS 1297..2424 /label=lacI-LVA misc_feature 1297..2424 /label=BBa_C0012 misc_feature 2425..2449 /label=Help:Barcodes CDS 1297..2424 /label=BBa_C0012 misc_feature 2462..2465 /label=conserved RBS 2458..2469 /label=BBa_B0034 misc_feature 2476..3195 /label=BBa_E0040 misc_feature 3190..3228 /label=LVA misc_feature 3229..3234 /label=Double Stop misc_feature 2476..2478 /label=Start misc_feature 2476..3234 /label=BBa_J04031 stem_loop 3254..3297 /label=stem_loop misc_feature 3243..3322 /label=BBa_B0010 terminator 3243..3322 /label=BBa_B0010 stem_loop 3338..3357 /label=T7 TE misc_feature 3364..3364 /label=stop polyA_site 3358..3371 /label=polya misc_feature 3331..3371 /label=BBa_B0012 terminator 3331..3371 /label=BBa_B0012 misc_feature 3378..4097 /label=BBa_E0040 misc_feature 4092..4130 /label=LVA misc_feature 4131..4136 /label=Double Stop misc_feature 3378..3380 /label=Start misc_feature 3378..4136 /label=BBa_J04031 stem_loop 4156..4199 /label=stem_loop misc_feature 4145..4224 /label=BBa_B0010 terminator 4145..4224 /label=BBa_B0010 stem_loop 4240..4259 /label=T7 TE misc_feature 4266..4266 /label=stop polyA_site 4260..4273 /label=polya misc_feature 4233..4273 /label=BBa_B0012 terminator 4233..4273 /label=BBa_B0012 ORIGIN 1 atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat 61 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa 121 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg 181 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg 241 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg 301 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa 361 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa 421 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac 481 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg 541 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt 601 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa 661 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc 721 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt 781 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa 841 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg 901 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 961 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 1021 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 1081 agcgggcagg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc 1141 tagtgtagat ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 1201 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 1261 caccttcggg tgggcctttc tgcgtttata tactagatgg tgaatgtgaa accagtaacg 1321 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 1381 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 1441 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 1501 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 1561 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 1621 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 1681 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 1741 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 1801 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 1861 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 1921 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 1981 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 2041 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt 2101 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 2161 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 2221 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 2281 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 2341 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa 2401 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagaaa 2461 gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt cccaattctt 2521 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt 2581 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt 2641 ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc gagataccca 2701 gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa 2761 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa 2821 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac 2881 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac 2941 aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga agatggaagc 3001 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc tgtcctttta 3061 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga 3121 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa 3181 ctatacaaaa ggcctgctgc aaacgacgaa aactacgctt tagtagctta ataatactag 3241 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 3301 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 3361 ctgcgtttat atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc 3421 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag 3481 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg 3541 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc 3601 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg 3661 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg 3721 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa 3781 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag 3841 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa 3901 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt 3961 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga 4021 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg 4081 aactatacaa aaggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact 4141 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 4201 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 4261 ttctgcgttt ata //