LOCUS       BBa_K079020             2165 bp    DNA     linear       09-May-2024
DEFINITION  GFP reporter under the control of J23118 promoter and Lac 2 
            operator auto-regulated by LacI protein
ACCESSION   BBa_K079020
VERSION     BBa_K079020.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23118
     promoter        44..64
                     /label=BBa_K079019
     misc_feature    77..80
                     /label=conserved
     RBS             73..84
                     /label=BBa_B0034
     misc_feature    1180..1218
                     /label=LVA
     CDS             91..1218
                     /label=lacI-LVA
     misc_feature    91..1218
                     /label=BBa_C0012
     misc_feature    1219..1243
                     /label=Help:Barcodes
     CDS             91..1218
                     /label=BBa_C0012
     misc_feature    73..1243
                     /label=BBa_S0100
     misc_feature    1256..1259
                     /label=conserved
     RBS             1252..1263
                     /label=BBa_B0034
     misc_feature    1270..1989
                     /label=BBa_E0040
     misc_feature    1984..2022
                     /label=LVA
     misc_feature    2023..2028
                     /label=Double Stop
     misc_feature    1270..1272
                     /label=Start
     misc_feature    1270..2028
                     /label=BBa_J04031
     stem_loop       2048..2091
                     /label=stem_loop
     misc_feature    2037..2116
                     /label=BBa_B0010
     terminator      2037..2116
                     /label=BBa_B0010
     stem_loop       2132..2151
                     /label=T7 TE
     misc_feature    2158..2158
                     /label=stop
     polyA_site      2152..2165
                     /label=polya
     misc_feature    2125..2165
                     /label=BBa_B0012
     terminator      2125..2165
                     /label=BBa_B0012
     misc_feature    1252..2165
                     /label=BBa_I763020
ORIGIN
        1 ttgacggcta gctcagtcct aggtattgtg ctagctacta gagaaatgtg agcgagtaac
       61 aacctactag agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac
      121 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc
      181 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac
      241 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc
      301 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc
      361 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt
      421 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg
      481 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt
      541 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg
      601 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc
      661 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc
      721 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa
      781 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat
      841 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat
      901 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc
      961 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc
     1021 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa
     1081 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     1141 cagctggcac gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac
     1201 gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga gaaagaggag
     1261 aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
     1321 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
     1381 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
     1441 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat
     1501 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
     1561 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
     1621 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
     1681 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
     1741 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
     1801 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
     1861 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     1921 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
     1981 aaaaggcctg ctgcaaacga cgaaaactac gctttagtag cttaataata ctagagccag
     2041 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2101 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     2161 ttata
//