LOCUS       BBa_K079026             2116 bp    DNA     linear       25-Nov-2024
DEFINITION  GFP reporter protein and LacI gene sequence under the  control of 
            J23118 constitutive promoter
ACCESSION   BBa_K079026
VERSION     BBa_K079026.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23118
     misc_feature    1131..1169
                     /label=LVA
     CDS             42..1169
                     /label=lacI-LVA
     misc_feature    42..1169
                     /label=BBa_C0012
     misc_feature    1170..1194
                     /label=Help:Barcodes
     CDS             42..1169
                     /label=BBa_C0012
     misc_feature    1207..1210
                     /label=conserved
     RBS             1203..1214
                     /label=BBa_B0034
     misc_feature    1221..1940
                     /label=BBa_E0040
     misc_feature    1935..1973
                     /label=LVA
     misc_feature    1974..1979
                     /label=Double Stop
     misc_feature    1221..1223
                     /label=Start
     misc_feature    1221..1979
                     /label=BBa_J04031
     stem_loop       1999..2042
                     /label=stem_loop
     misc_feature    1988..2067
                     /label=BBa_B0010
     terminator      1988..2067
                     /label=BBa_B0010
     stem_loop       2083..2102
                     /label=T7 TE
     misc_feature    2109..2109
                     /label=stop
     polyA_site      2103..2116
                     /label=polya
     misc_feature    2076..2116
                     /label=BBa_B0012
     terminator      2076..2116
                     /label=BBa_B0012
     misc_feature    1203..2116
                     /label=BBa_I763020
ORIGIN
        1 ttgacggcta gctcagtcct aggtattgtg ctagctacta gatggtgaat gtgaaaccag
       61 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
      121 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
      181 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
      241 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta
      301 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
      361 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca
      421 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc
      481 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
      541 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
      601 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
      661 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
      721 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg
      781 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
      841 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
      901 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
      961 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     1021 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     1081 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag gctgcaaacg
     1141 acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag
     1201 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
     1261 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
     1321 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
     1381 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
     1441 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
     1501 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
     1561 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
     1621 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
     1681 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
     1741 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
     1801 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
     1861 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
     1921 atgaactata caaaaggcct gctgcaaacg acgaaaacta cgctttagta gcttaataat
     1981 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     2041 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     2101 cctttctgcg tttata
//