##gff-version 3
##sequence-region BBa_K079027 1 2501
BBa_K079027	.	ribosome_entry_site	1588	1599	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K079027	.	conserved_region	1592	1595	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K079027	.	promoter	1	35	.	+	0	ID=BBa_J23114;Name=BBa_J23114
BBa_K079027	.	CDS	62	1189	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K079027	.	engineered_tag	1190	1214	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K079027	.	tag	1151	1189	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K079027	.	engineered_region	62	1189	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K079027	.	CDS	62	1189	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K079027	.	terminator	1311	1351	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K079027	.	engineered_region	1311	1351	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K079027	.	polyA_site	1338	1351	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K079027	.	stop_codon	1344	1344	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K079027	.	stem_loop	1318	1337	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K079027	.	terminator	2461	2501	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K079027	.	engineered_region	2461	2501	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K079027	.	polyA_site	2488	2501	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K079027	.	stop_codon	2494	2494	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K079027	.	stem_loop	2468	2487	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K079027	.	terminator	1223	1302	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K079027	.	engineered_region	1223	1302	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K079027	.	stem_loop	1234	1277	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K079027	.	promoter	1360	1559	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K079027	.	CDS	1360	1447	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K079027	.	engineered_region	1360	1559	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K079027	.	non_covalent_binding_site	1448	1485	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K079027	.	start_codon	1532	1532	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K079027	.	non_covalent_binding_site	1525	1559	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K079027	.	promoter	1520	1525	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K079027	.	promoter	1496	1501	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K079027	.	ribosome_entry_site	1568	1579	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K079027	.	conserved_region	1572	1575	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K079027	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K079027	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K079027	.	engineered_region	1606	2364	.	+	0	ID=BBa_J04031;Name=BBa_J04031
BBa_K079027	.	tag	2320	2358	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_K079027	.	stop_codon	2359	2364	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_K079027	.	start_codon	1606	1608	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_K079027	.	engineered_region	1606	2325	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_K079027	.	terminator	2373	2452	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K079027	.	engineered_region	2373	2452	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K079027	.	stem_loop	2384	2427	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K079027
tttatggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatggtgaatgtgaaacca
gtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacg
tttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaact
ggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcg
attaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaag
cggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgc
tgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttc
tcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcc
cattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagc
ggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccact
gcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcgg
atatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattt
tcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttg
cccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcat
taatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggt
ttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttaca
ctttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaat
actagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaatta
gatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctta
aatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctt
tgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaact
atatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcg
agttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgt
atacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgtt
caactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcca
cacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggat
tacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactag
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata