LOCUS       BBa_K079028             2501 bp    DNA     linear       08-May-2024
DEFINITION  ...
ACCESSION   BBa_K079028
VERSION     BBa_K079028.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23105
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1151..1189
                     /label=LVA
     CDS             62..1189
                     /label=lacI-LVA
     misc_feature    62..1189
                     /label=BBa_C0012
     misc_feature    1190..1214
                     /label=Help:Barcodes
     CDS             62..1189
                     /label=BBa_C0012
     stem_loop       1234..1277
                     /label=stem_loop
     misc_feature    1223..1302
                     /label=BBa_B0010
     terminator      1223..1302
                     /label=BBa_B0010
     stem_loop       1318..1337
                     /label=T7 TE
     misc_feature    1344..1344
                     /label=stop
     polyA_site      1338..1351
                     /label=polya
     misc_feature    1311..1351
                     /label=BBa_B0012
     terminator      1311..1351
                     /label=BBa_B0012
     CDS             1360..1447
                     /label=end of LacI coding region (inactive)
     misc_feature    1360..1559
                     /label=BBa_R0010
     misc_feature    1448..1485
                     /label=CAP binding site
     promoter        1496..1501
                     /label=-35
     promoter        1520..1525
                     /label=-10
     misc_feature    1525..1559
                     /label=LacI binding site
     misc_feature    1532..1532
                     /label=start
     promoter        1360..1559
                     /label=BBa_R0010
     misc_feature    1572..1575
                     /label=conserved
     RBS             1568..1579
                     /label=BBa_B0034
     misc_feature    1592..1595
                     /label=conserved
     RBS             1588..1599
                     /label=BBa_B0034
     misc_feature    1606..2325
                     /label=BBa_E0040
     misc_feature    2320..2358
                     /label=LVA
     misc_feature    2359..2364
                     /label=Double Stop
     misc_feature    1606..1608
                     /label=Start
     misc_feature    1606..2364
                     /label=BBa_J04031
     stem_loop       2384..2427
                     /label=stem_loop
     misc_feature    2373..2452
                     /label=BBa_B0010
     terminator      2373..2452
                     /label=BBa_B0010
     stem_loop       2468..2487
                     /label=T7 TE
     misc_feature    2494..2494
                     /label=stop
     polyA_site      2488..2501
                     /label=polya
     misc_feature    2461..2501
                     /label=BBa_B0012
     terminator      2461..2501
                     /label=BBa_B0012
ORIGIN
        1 tttacggcta gctcagtcct aggtactatg ctagctacta gagaaagagg agaaatacta
       61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
      601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
      721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
      781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
      841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
      901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
      961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1321 tcaccttcgg gtgggccttt ctgcgtttat atactagagc aatacgcaaa ccgcctctcc
     1381 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     1441 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     1501 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacat
     1561 actagagaaa gaggagaaat actagagaaa gaggagaaat actagatgcg taaaggagaa
     1621 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
     1681 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
     1741 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt
     1801 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag
     1861 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
     1921 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
     1981 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
     2041 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc
     2101 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
     2161 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
     2221 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
     2281 gctgctggga ttacacatgg catggatgaa ctatacaaaa ggcctgctgc aaacgacgaa
     2341 aactacgctt tagtagctta ataatactag agccaggcat caaataaaac gaaaggctca
     2401 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     2461 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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