LOCUS       BBa_K079029             2481 bp    DNA     linear       09-May-2024
DEFINITION  GFP expression controlled by LacI repressor under the BBa_J23114 
            promoter
ACCESSION   BBa_K079029
VERSION     BBa_K079029.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23114
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1151..1189
                     /label=LVA
     CDS             62..1189
                     /label=lacI-LVA
     misc_feature    62..1189
                     /label=BBa_C0012
     misc_feature    1190..1214
                     /label=Help:Barcodes
     CDS             62..1189
                     /label=BBa_C0012
     misc_feature    44..1214
                     /label=BBa_S0100
     stem_loop       1234..1277
                     /label=stem_loop
     misc_feature    1223..1302
                     /label=BBa_B0010
     terminator      1223..1302
                     /label=BBa_B0010
     stem_loop       1318..1337
                     /label=T7 TE
     misc_feature    1344..1344
                     /label=stop
     polyA_site      1338..1351
                     /label=polya
     misc_feature    1311..1351
                     /label=BBa_B0012
     terminator      1311..1351
                     /label=BBa_B0012
     terminator      1223..1351
                     /label=BBa_B0015
     CDS             1360..1447
                     /label=end of LacI coding region (inactive)
     misc_feature    1360..1559
                     /label=BBa_R0010
     misc_feature    1448..1485
                     /label=CAP binding site
     promoter        1496..1501
                     /label=-35
     promoter        1520..1525
                     /label=-10
     misc_feature    1525..1559
                     /label=LacI binding site
     misc_feature    1532..1532
                     /label=start
     promoter        1360..1559
                     /label=BBa_R0010
     misc_feature    1572..1575
                     /label=conserved
     RBS             1568..1579
                     /label=BBa_B0034
     misc_feature    1586..2305
                     /label=BBa_E0040
     misc_feature    2300..2338
                     /label=LVA
     misc_feature    2339..2344
                     /label=Double Stop
     misc_feature    1586..1588
                     /label=Start
     misc_feature    1586..2344
                     /label=BBa_J04031
     stem_loop       2364..2407
                     /label=stem_loop
     misc_feature    2353..2432
                     /label=BBa_B0010
     terminator      2353..2432
                     /label=BBa_B0010
     stem_loop       2448..2467
                     /label=T7 TE
     misc_feature    2474..2474
                     /label=stop
     polyA_site      2468..2481
                     /label=polya
     misc_feature    2441..2481
                     /label=BBa_B0012
     terminator      2441..2481
                     /label=BBa_B0012
     misc_feature    1568..2481
                     /label=BBa_I763020
ORIGIN
        1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta
       61 gatggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
      121 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
      181 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
      241 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
      301 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
      361 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
      421 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
      481 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
      541 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
      601 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
      661 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
      721 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
      781 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
      841 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
      901 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
      961 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     1021 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     1081 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     1141 aagcgggcag gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1201 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1261 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1321 tcaccttcgg gtgggccttt ctgcgtttat atactagagc aatacgcaaa ccgcctctcc
     1381 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     1441 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     1501 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacat
     1561 actagagaaa gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt
     1621 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
     1681 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
     1741 actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc
     1801 gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta
     1861 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt
     1921 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
     1981 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat
     2041 catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga
     2101 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
     2161 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
     2221 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
     2281 catggatgaa ctatacaaaa ggcctgctgc aaacgacgaa aactacgctt tagtagctta
     2341 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2401 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     2461 gtgggccttt ctgcgtttat a
//