LOCUS BBa_K079021 2165 bp DNA linear 26-Nov-2024 DEFINITION LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La ACCESSION BBa_K079021 VERSION BBa_K079021.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23118 promoter 44..64 /label=BBa_K079018 misc_feature 77..80 /label=conserved RBS 73..84 /label=BBa_B0034 misc_feature 1180..1218 /label=LVA CDS 91..1218 /label=lacI-LVA misc_feature 91..1218 /label=BBa_C0012 misc_feature 1219..1243 /label=Help:Barcodes CDS 91..1218 /label=BBa_C0012 misc_feature 73..1243 /label=BBa_S0100 misc_feature 1256..1259 /label=conserved RBS 1252..1263 /label=BBa_B0034 misc_feature 1270..1989 /label=BBa_E0040 misc_feature 1984..2022 /label=LVA misc_feature 2023..2028 /label=Double Stop misc_feature 1270..1272 /label=Start misc_feature 1270..2028 /label=BBa_J04031 stem_loop 2048..2091 /label=stem_loop misc_feature 2037..2116 /label=BBa_B0010 terminator 2037..2116 /label=BBa_B0010 stem_loop 2132..2151 /label=T7 TE misc_feature 2158..2158 /label=stop polyA_site 2152..2165 /label=polya misc_feature 2125..2165 /label=BBa_B0012 terminator 2125..2165 /label=BBa_B0012 misc_feature 1252..2165 /label=BBa_I763020 ORIGIN 1 ttgacggcta gctcagtcct aggtattgtg ctagctacta gagaattgtg agcggataac 61 aatttactag agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac 121 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc 181 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac 241 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc 301 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc 361 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt 421 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg 481 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt 541 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg 601 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc 661 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc 721 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa 781 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat 841 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat 901 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc 961 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc 1021 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa 1081 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 1141 cagctggcac gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac 1201 gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga gaaagaggag 1261 aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa 1321 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca 1381 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg 1441 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat 1501 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact 1561 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat 1621 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt 1681 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa 1741 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa 1801 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac 1861 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac 1921 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac 1981 aaaaggcctg ctgcaaacga cgaaaactac gctttagtag cttaataata ctagagccag 2041 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2101 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 2161 ttata // LOCUS BBa_K079051 2165 bp DNA linear 26-Nov-2024 DEFINITION LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator ACCESSION BBa_K079051 VERSION BBa_K079051.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23118 promoter 44..64 /label=BBa_K079018 misc_feature 77..80 /label=conserved RBS 73..84 /label=BBa_B0034 misc_feature 1180..1218 /label=LVA CDS 91..1218 /label=lacI-LVA misc_feature 91..1218 /label=BBa_C0012 misc_feature 1219..1243 /label=Help:Barcodes CDS 91..1218 /label=BBa_C0012 misc_feature 1256..1259 /label=conserved RBS 1252..1263 /label=BBa_B0034 misc_feature 1270..1989 /label=BBa_E0040 misc_feature 1984..2022 /label=LVA misc_feature 2023..2028 /label=Double Stop misc_feature 1270..1272 /label=Start misc_feature 1270..2028 /label=BBa_J04031 stem_loop 2048..2091 /label=stem_loop misc_feature 2037..2116 /label=BBa_B0010 terminator 2037..2116 /label=BBa_B0010 stem_loop 2132..2151 /label=T7 TE misc_feature 2158..2158 /label=stop polyA_site 2152..2165 /label=polya misc_feature 2125..2165 /label=BBa_B0012 terminator 2125..2165 /label=BBa_B0012 ORIGIN 1 ttgacggcta gctcagtcct aggtattgtg ctagctacta gagaattgtg agcggataac 61 aatttactag agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac 121 gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc 181 agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac 241 attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc 301 acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc 361 gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt 421 aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg 481 ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt 541 cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg 601 cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc 661 ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc 721 aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa 781 caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat 841 cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat 901 atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc 961 accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc 1021 tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa 1081 accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 1141 cagctggcac gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac 1201 gctttagtag cttaataact ctgatagtgc tagtgtagat ctctactaga gaaagaggag 1261 aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa 1321 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca 1381 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg 1441 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat 1501 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact 1561 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat 1621 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt 1681 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa 1741 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa 1801 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac 1861 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac 1921 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac 1981 aaaaggcctg ctgcaaacga cgaaaactac gctttagtag cttaataata ctagagccag 2041 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 2101 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 2161 ttata //