LOCUS       BBa_K079052             2164 bp    DNA     linear       07-May-2025
DEFINITION  LacI repressor and GFP reporter proteins controlled by the J23118 
            promoter and Lac symmetric operato
ACCESSION   BBa_K079052
VERSION     BBa_K079052.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23118
     promoter        44..63
                     /label=BBa_K079017
     misc_feature    76..79
                     /label=conserved
     RBS             72..83
                     /label=BBa_B0034
     misc_feature    1179..1217
                     /label=LVA
     CDS             90..1217
                     /label=lacI-LVA
     misc_feature    90..1217
                     /label=BBa_C0012
     misc_feature    1218..1242
                     /label=Help:Barcodes
     CDS             90..1217
                     /label=BBa_C0012
     misc_feature    1255..1258
                     /label=conserved
     RBS             1251..1262
                     /label=BBa_B0034
     misc_feature    1269..1988
                     /label=BBa_E0040
     misc_feature    1983..2021
                     /label=LVA
     misc_feature    2022..2027
                     /label=Double Stop
     misc_feature    1269..1271
                     /label=Start
     misc_feature    1269..2027
                     /label=BBa_J04031
     stem_loop       2047..2090
                     /label=stem_loop
     misc_feature    2036..2115
                     /label=BBa_B0010
     terminator      2036..2115
                     /label=BBa_B0010
     stem_loop       2131..2150
                     /label=T7 TE
     misc_feature    2157..2157
                     /label=stop
     polyA_site      2151..2164
                     /label=polya
     misc_feature    2124..2164
                     /label=BBa_B0012
     terminator      2124..2164
                     /label=BBa_B0012
ORIGIN
        1 ttgacggcta gctcagtcct aggtattgtg ctagctacta gagaattgtg agcgctcaca
       61 atttactaga gaaagaggag aaatactaga tggtgaatgt gaaaccagta acgttatacg
      121 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
      181 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
      241 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
      301 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
      361 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
      421 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
      481 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
      541 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
      601 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
      661 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
      721 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
      781 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
      841 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
      901 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
      961 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     1021 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     1081 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     1141 agctggcacg acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg
     1201 ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag aaagaggaga
     1261 aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     1321 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     1381 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
     1441 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata
     1501 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
     1561 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
     1621 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
     1681 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
     1741 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     1801 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     1861 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     1921 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     1981 aaaggcctgc tgcaaacgac gaaaactacg ctttagtagc ttaataatac tagagccagg
     2041 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2101 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     2161 tata
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