BBa_K079052
type: ComponentDefinition
persistent identity: BBa K079052
display id: BBa_K079052
version: 1
was generated by: iGEM to SBOL conversion
description: LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato
created: 2008-10-29T12:00:00Z
modified: 2015-05-08T01:08:34Z
mutable provenance: ..
owned by: james
owned by: myers
mutable description: ..
discontinued: true
dominant: false
experience: experience/Issues
group u list: _185_
m user id: 0
owner id: 3530
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: true
mutable notes: ..
star: false
creator: Francesca Ceroni
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_J04031
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
component: BBa_K079017
component: BBa_J23118
component: BBa_C0012
sequence annotation: BBa_J23118
sequence annotation: BBa_K079017
sequence annotation: BBa_B0034
sequence annotation: BBa_C0012
sequence annotation: BBa_J04031
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K079052_sequence/1

BBa_J04031
type: Component
persistent identity: component1995639
display id: component1995639
version: 1
top level: BBa_K079052
definition: BBa_J04031
access: public

BBa_B0034
type: Component
persistent identity: component1995634
display id: component1995634
version: 1
top level: BBa_K079052
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component1995642
display id: component1995642
version: 1
top level: BBa_K079052
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1995640
display id: component1995640
version: 1
top level: BBa_K079052
definition: BBa_B0010
access: public

BBa_K079017
type: Component
persistent identity: component1995626
display id: component1995626
version: 1
top level: BBa_K079052
definition: Lac operat
access: public

BBa_J23118
type: Component
persistent identity: component1995625
display id: component1995625
version: 1
top level: BBa_K079052
definition: BBa_J23118
access: public

BBa_C0012
type: Component
persistent identity: component1995630
display id: component1995630
version: 1
top level: BBa_K079052
definition: lacI
access: public

BBa_B0034
type: Component
persistent identity: component1995628
display id: component1995628
version: 1
top level: BBa_K079052
definition: BBa_B0034
access: public

BBa_J23118
type: SequenceAnnotation
persistent identity: annotation1995625
display id: annotation1995625
version: 1
top level: BBa_K079052
location: https://synbiohub.org/public/igem/BBa_K079052/annotation1995625/range1995625/1
component: BBa_J23118

https://synbiohub.org/public/igem/BBa_K079052/annotation1995625/range1995625/1
type: Range
persistent identity: range1995625
display id: range1995625
version: 1
top level: BBa_K079052
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K079017
type: SequenceAnnotation
persistent identity: annotation1995626
display id: annotation1995626
version: 1
top level: BBa_K079052
location: https://synbiohub.org/public/igem/BBa_K079052/annotation1995626/range1995626/1
component: BBa_K079017

https://synbiohub.org/public/igem/BBa_K079052/annotation1995626/range1995626/1
type: Range
persistent identity: range1995626
display id: range1995626
version: 1
top level: BBa_K079052
direction: direction/reverse
start: 44
end: 63
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1995628
display id: annotation1995628
version: 1
top level: BBa_K079052
location: https://synbiohub.org/public/igem/BBa_K079052/annotation1995628/range1995628/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K079052/annotation1995628/range1995628/1
type: Range
persistent identity: range1995628
display id: range1995628
version: 1
top level: BBa_K079052
direction: direction/reverse
start: 72
end: 83
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1995630
display id: annotation1995630
version: 1
top level: BBa_K079052
location: https://synbiohub.org/public/igem/BBa_K079052/annotation1995630/range1995630/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_K079052/annotation1995630/range1995630/1
type: Range
persistent identity: range1995630
display id: range1995630
version: 1
top level: BBa_K079052
direction: direction/forward
start: 90
end: 1217
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1995634
display id: annotation1995634
version: 1
top level: BBa_K079052
location: https://synbiohub.org/public/igem/BBa_K079052/annotation1995634/range1995634/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K079052/annotation1995634/range1995634/1
type: Range
persistent identity: range1995634
display id: range1995634
version: 1
top level: BBa_K079052
direction: direction/reverse
start: 1251
end: 1262
orientation: inline

BBa_J04031
type: SequenceAnnotation
persistent identity: annotation1995639
display id: annotation1995639
version: 1
top level: BBa_K079052
location: https://synbiohub.org/public/igem/BBa_K079052/annotation1995639/range1995639/1
component: BBa_J04031

https://synbiohub.org/public/igem/BBa_K079052/annotation1995639/range1995639/1
type: Range
persistent identity: range1995639
display id: range1995639
version: 1
top level: BBa_K079052
direction: direction/reverse
start: 1269
end: 2027
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1995640
display id: annotation1995640
version: 1
top level: BBa_K079052
location: https://synbiohub.org/public/igem/BBa_K079052/annotation1995640/range1995640/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K079052/annotation1995640/range1995640/1
type: Range
persistent identity: range1995640
display id: range1995640
version: 1
top level: BBa_K079052
direction: direction/forward
start: 2036
end: 2115
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1995642
display id: annotation1995642
version: 1
top level: BBa_K079052
location: https://synbiohub.org/public/igem/BBa_K079052/annotation1995642/range1995642/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K079052/annotation1995642/range1995642/1
type: Range
persistent identity: range1995642
display id: range1995642
version: 1
top level: BBa_K079052
direction: direction/forward
start: 2124
end: 2164
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

BBa_J04031
type: ComponentDefinition
persistent identity: BBa J04031
display id: BBa_J04031
version: 1
was generated by: iGEM to SBOL conversion
description: GFP w/LVA tag
created: 2005-06-07T11:00:00Z
modified: 2015-08-31T04:08:13Z
mutable provenance: Davidson Synth Aces
owned by: james
owned by: myers
mutable description: BBa_E0040 with an LVA tag added via PCR. BBa_E0040 is GFP(mut3b).
discontinued: false
dominant: true
experience: experience/None
group u list: _16_
m user id: 0
owner id: 326
owning group id: 16
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Kristen DeCelle
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: BBa_E0040
sequence annotation: Start
sequence annotation: LVA
sequence annotation: Double Stop
sequence: https://synbiohub.org/public/igem/BBa_J04031_sequence/1

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1507202
display id: annotation1507202
version: 1
top level: BBa_J04031
location: https://synbiohub.org/public/igem/BBa_J04031/annotation1507202/range1507202/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_J04031/annotation1507202/range1507202/1
type: Range
persistent identity: range1507202
display id: range1507202
version: 1
top level: BBa_J04031
direction: direction/forward
start: 1
end: 720
orientation: inline

Start
type: SequenceAnnotation
persistent identity: annotation1507246
display id: annotation1507246
version: 1
top level: BBa_J04031
location: https://synbiohub.org/public/igem/BBa_J04031/annotation1507246/range1507246/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_J04031/annotation1507246/range1507246/1
type: Range
persistent identity: range1507246
display id: range1507246
version: 1
top level: BBa_J04031
direction: direction/forward
start: 1
end: 3
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1507235
display id: annotation1507235
version: 1
top level: BBa_J04031
location: https://synbiohub.org/public/igem/BBa_J04031/annotation1507235/range1507235/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_J04031/annotation1507235/range1507235/1
type: Range
persistent identity: range1507235
display id: range1507235
version: 1
top level: BBa_J04031
direction: direction/forward
start: 715
end: 753
orientation: inline

Double Stop
type: SequenceAnnotation
persistent identity: annotation1507242
display id: annotation1507242
version: 1
top level: BBa_J04031
location: https://synbiohub.org/public/igem/BBa_J04031/annotation1507242/range1507242/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_J04031/annotation1507242/range1507242/1
type: Range
persistent identity: range1507242
display id: range1507242
version: 1
top level: BBa_J04031
direction: direction/forward
start: 754
end: 759
orientation: inline

Lac operat
type: ComponentDefinition
persistent identity: BBa K079017
display id: BBa_K079017
version: 1
was generated by: iGEM to SBOL conversion
description: Lac symmetric - operator library member
created: 2008-10-23T11:00:00Z
modified: 2015-05-08T01:08:33Z
mutable provenance: Geneart synthesis service.
owned by: james
owned by: myers
mutable description: The Lac operator sequences
discontinued: false
dominant: false
experience: experience/Works
group u list: _185_
m user id: 0
owner id: 3530
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: true
mutable notes: The BBa_K079017 part was designed as the Lac symmetric operator sequence flanked by the standard Prefix and Suffix.
star: false
creator: Francesca Ceroni
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K079017_sequence/1

BBa_J23118
type: ComponentDefinition
persistent identity: BBa J23118
display id: BBa_J23118
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23118_sequence/1

https://synbiohub.org/public/igem/BBa_K079052_sequence/1
type: Sequence
persistent identity: BBa K079052 sequence
display id: BBa_K079052_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtattgtgctagctactagagaattgtgagcgctcacaatttactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J04031_sequence/1
type: Sequence
persistent identity: BBa J04031 sequence
display id: BBa_J04031_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K079017_sequence/1
type: Sequence
persistent identity: BBa K079017 sequence
display id: BBa_K079017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcgctcacaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23118_sequence/1
type: Sequence
persistent identity: BBa J23118 sequence
display id: BBa_J23118_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtattgtgctagc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z