BBa_K080000 1 BBa_K080000 TCFbs-BMP4 2008-10-19T11:00:00Z 2015-05-08T01:08:34Z TCFbs minPro was isolated via PCR from a human lung embryonal carcinoma cell line, NT2 genomic DNA (atcc # CRL-1973). ???Gaire, M., et al. Structure and expression of the human gene for the matrix metalloproteinase matrilysn. (1994) JBC 269:3 2032-2040??? From previous studies, we found a minimal promoter sequence where Beta-catenin/tcf4 could bind and control the expression of MMP7 ???Thomas Brabletz, Andreas Jung, Serpil Dag, Falk Hlubek, and Thomas Kirchner. β-Catenin Regulates the Expression of the Matrix Metalloproteinase-7 in Human Colorectal Cancer. 1999. American J. of Path. 155(4):1033-1038??? BMP4 Mus musculus full length cDNA clone from openbiosystems, clone ID# 4219098 Induction of BMP4 during early stages of cardiomyocyte differentiation results in inhibitory effects. From previous studies it is not clear whether BMP4 is necessary for cardiomyocyte differentiation. In our circuit, nkx2.5 and gata4 will be induced and initiate the pathways of cardiac differentiation. During normal cardiac muscle differentiation BMP4 is a downstream target of nkx-2.5 and gata4, the expression of which turns on at later stages of cardiac differentiation. To ensure that BMP4 has a delayed expression, we designed BMP4 under the control of minimal promoters of late markers of cardiac muscle differentiation, TCF and alpha cardiac actin. false false _182_ 0 3648 9 Not in stock false None false Jennifer Lei BBa_K080000_sequence 1 agagagatcgattctagaataatgtcctgaatgatacctatgagagcagtcatttgactttggcaaaaaaatgaggtttctcatggagtcaatttatgcagcagacagaaaaaaaaatcctttgaaagacaaatacattgtgtgcttcctgccaataacgatgtaatacttcctcgttttagttaatgaaaaataacacatactttcaaagttctgtagactctaaaaagaaagaaaacactcaaatgagtcacctatttccacattcgaggctgagaagctatataaatttctgcagtcactagcagaaaacattaattaaacgcgtgccaccatgattcctggtaaccgaatgctgatggtcgttttattatgccaagtcctgctaggaggcgcgagccatgctagtttgatacctgagaccgggaagaaaaaagtcgccgagattcagggccacgcgggaggacgccgctcagggcagagccatgagctcctgcgggacttcgaggcgacacttctacagatgtttgggctgcgccgccgtccgcagcctagcaagagcgccgtcattccggattacatgagggatctttaccggctccagtctggggaggaggaggaggaagagcagagccagggaaccgggcttgagtacccggagcgtcccgccagccgagccaacactgtgaggagtttccatcacgaagaacatctggagaacatcccagggaccagtgagagctctgcttttcgtttcctcttcaacctcagcagcatcccagaaaatgaggtgatctcctcggcagagctccggctctttcgggagcaggtggaccagggccctgactgggaacagggcttccaccgtataaacatttatgaggttatgaagcccccagcagaaatggttcctggacacctcatcacacgactactggacaccagactagtccatcacaatgtgacacggtgggaaactttcgatgtgagccctgcagtccttcgctggacccgggaaaagcaacccaattatgggctggccattgaggtgactcacctccaccagacacggacccaccagggccagcatgtcagaatcagccgatcgttacctcaagggagtggagattgggcccaactccggcccctcctggtcacttttggccatgatggccggggccataccttgacccgcaggagggccaaacgtagtcccaagcatcacccacagcggtccaggaagaagaataagaactgccgtcgccattcactatacgtggacttcagtgacgtgggctggaatgattggattgtggccccacccggctaccaggccttctactgccatggggactgtccctttccactggctgatcacctcaactcaaccaaccatgccattgtgcagaccctagtcaactctgttaattctagtatccctaaggcctgttgtgtccccactgaactgagtgccatttccatgttgtacctggatgagtatgacaaggtggtgttgaaaaattatcaggagatggtggtagaggggtgtggatgccgctgataggctagcgtcgacctctct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z