##gff-version 3
##sequence-region BBa_K081000 1 774
BBa_K081000	.	terminator	673	711	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K081000	.	polyA_site	704	711	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K081000	.	polyA_site	673	681	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K081000	.	engineered_region	673	711	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K081000	.	stem_loop	682	703	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K081000	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K081000	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K081000	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K081000	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K081000	.	CDS	22	639	.	+	0	ID=BBa_C0061;Name=BBa_C0061
BBa_K081000	.	engineered_region	22	639	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K081000	.	tag	601	632	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K081000	.	engineered_tag	640	664	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K081000	.	CDS	22	600	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K081000	.	promoter	720	774	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K081000	.	non_covalent_binding_site	720	739	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K081000	.	promoter	761	766	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K081000	.	engineered_region	720	774	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K081000	.	start_codon	772	772	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K081000	.	promoter	739	744	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
>BBa_K081000
attaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaag
gtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcaga
tgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttatta
cctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaata
tagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaact
atttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcga
tttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttg
tattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagc
ttaataactctgatagtgctagtgtagatctctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
tactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa