LOCUS BBa_K081004 2630 bp DNA linear 22-Nov-2024 DEFINITION Demux SEL ACCESSION BBa_K081004 VERSION BBa_K081004.1 FEATURES Location/Qualifiers RBS 1..15 /label=RBS-1\Strong RBS 8..12 /label=RBS misc_feature 1..15 /label=BBa_B0030 RBS 1..15 /label=BBa_B0030 CDS 25..732 /label=cI lambda misc_feature 733..765 /label=LVA misc_feature 772..796 /label=Help:Barcodes CDS 22..796 /label=BBa_C0051 RBS 805..819 /label=RBS-1\Strong RBS 812..816 /label=RBS misc_feature 805..819 /label=BBa_B0030 RBS 805..819 /label=BBa_B0030 CDS 826..1542 /label=lasR misc_feature 1543..1575 /label=LVA misc_feature 1305..1305 /label=G misc_feature 1582..1606 /label=Help:Barcodes CDS 826..1606 /label=BBa_C0079 misc_feature 1615..1653 /label=B1006 stem_loop 1624..1645 /label=modified thr terminator polyA_site 1646..1653 /label=PolyA polyA_site 1615..1623 /label=PolyA terminator 1615..1653 /label=BBa_B1006 promoter 1699..1704 /label=-10 promoter 1676..1681 /label=-35 misc_feature 1686..1702 /label=OR1 misc_feature 1662..1678 /label=OR2 misc_feature 1662..1710 /label=BBa_R0051 promoter 1662..1710 /label=BBa_R0051 RBS 1719..1733 /label=RBS-1\Strong RBS 1726..1730 /label=RBS misc_feature 1719..1733 /label=BBa_B0030 RBS 1719..1733 /label=BBa_B0030 misc_feature 1740..1741 /label=prefix misc_feature 1913..1913 /label=T misc_feature 2231..2231 /label=A CDS 1740..2489 /label=luxR misc_feature 1740..2495 /label=BBa_C0062 misc_feature 2496..2520 /label=Help:Barcodes CDS 1740..2495 /label=BBa_C0062 misc_feature 2529..2567 /label=B1006 stem_loop 2538..2559 /label=modified thr terminator polyA_site 2560..2567 /label=PolyA polyA_site 2529..2537 /label=PolyA terminator 2529..2567 /label=BBa_B1006 misc_feature 2576..2595 /label=LuxR/HSL promoter 2595..2600 /label=-35 promoter 2617..2622 /label=-10 misc_feature 2628..2628 /label=start misc_feature 2576..2630 /label=BBa_R0062 promoter 2576..2630 /label=BBa_R0062 ORIGIN 1 attaaagagg agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt 61 gaggacgcac gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc 121 caggaatctg tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat 181 ggcatcaatg cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc 241 gttgaagaat ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt 301 atgcagccgt cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg 361 atgttctcac ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca 421 accaaaaaag ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca 481 ccaacaggct ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag 541 gctgttgagc caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag 601 aaactgatca gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg 661 atcccatgca atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa 721 gagacgtttg gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag 781 tgctagtgta gatcgctact agagattaaa gaggagaaat actagatggc cttggttgac 841 ggttttcttg agctggaacg ctcaagtgga aaattggagt ggagcgccat cctccagaag 901 atggcgagcg accttggatt ctcgaagatc ctgttcggcc tgttgcctaa ggacagccag 961 gactacgaga acgccttcat cgtcggcaac tacccggccg cctggcgcga gcattacgac 1021 cgggctggct acgcgcgggt cgacccgacg gtcagtcact gtacccagag cgtactgccg 1081 attttctggg aaccgtccat ctaccagacg cgaaagcagc acgagttctt cgaggaagcc 1141 tcggccgccg gcctggtgta tgggctgacc atgccgctgc atggtgctcg cggcgaactc 1201 ggcgcgctga gcctcagcgt ggaagcggaa aaccgggccg aggccaaccg tttcatagag 1261 tcggtcctgc cgaccctgtg gatgctcaag gactacgcac tgcaaagcgg tgccggactg 1321 gccttcgaac atccggtcag caaaccggtg gttctgacca gccgggagaa ggaagtgttg 1381 cagtggtgcg ccatcggcaa gaccagttgg gagatatcgg ttatctgcaa ctgctcggaa 1441 gccaatgtga acttccatat gggaaatatt cggcggaagt tcggtgtgac ctcccgccgc 1501 gtagcggcca ttatggccgt taatttgggt cttattactc tcgctgcaaa cgacgaaaac 1561 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagaaaaaa 1621 aaaccccgcc cctgacaggg cggggttttt ttttactaga gtaacaccgt gcgtgttgac 1681 tattttacct ctggcggtga taatggttgc tactagagat taaagaggag aaatactaga 1741 tgaaaaacat aaatgccgac gacacataca gaataattaa taaaattaaa gcttgtagaa 1801 gcaataatga tattaatcaa tgcttatctg atatgactaa aatggtacat tgtgaatatt 1861 atttactcgc gatcatttat cctcattcta tggttaaatc tgatatttca atcctagata 1921 attaccctaa aaaatggagg caatattatg atgacgctaa tttaataaaa tatgatccta 1981 tagtagatta ttctaactcc aatcattcac caattaattg gaatatattt gaaaacaatg 2041 ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa aacatcaggt cttatcactg 2101 ggtttagttt ccctattcat acggctaaca atggcttcgg aatgcttagt tttgcacatt 2161 cagaaaaaga caactatata gatagtttat ttttacatgc gtgtatgaac ataccattaa 2221 ttgttccttc tctagttgat aattatcgaa aaataaatat agcaaataat aaatcaaaca 2281 acgatttaac caaaagagaa aaagaatgtt tagcgtgggc atgcgaagga aaaagctctt 2341 gggatatttc aaaaatatta ggttgcagtg agcgtactgt cactttccat ttaaccaatg 2401 cgcaaatgaa actcaataca acaaaccgct gccaaagtat ttctaaagca attttaacag 2461 gagcaattga ttgcccatac tttaaaaatt aataacactg atagtgctag tgtagatcac 2521 tactagagaa aaaaaaaccc cgcccctgac agggcggggt ttttttttac tagagacctg 2581 taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataaa //