##gff-version 3
##sequence-region BBa_K081004 1 2630
BBa_K081004	.	ribosome_entry_site	1719	1733	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K081004	.	engineered_region	1719	1733	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K081004	.	ribosome_entry_site	1726	1730	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K081004	.	ribosome_entry_site	1719	1733	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K081004	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K081004	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K081004	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K081004	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K081004	.	promoter	1662	1710	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K081004	.	promoter	1699	1704	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
BBa_K081004	.	promoter	1676	1681	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K081004	.	engineered_region	1662	1710	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K081004	.	non_covalent_binding_site	1686	1702	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K081004	.	non_covalent_binding_site	1662	1678	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K081004	.	terminator	2529	2567	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K081004	.	polyA_site	2560	2567	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K081004	.	engineered_region	2529	2567	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K081004	.	stem_loop	2538	2559	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K081004	.	polyA_site	2529	2537	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K081004	.	ribosome_entry_site	805	819	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K081004	.	engineered_region	805	819	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K081004	.	ribosome_entry_site	812	816	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K081004	.	ribosome_entry_site	805	819	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K081004	.	CDS	826	1606	.	+	0	ID=BBa_C0079;Name=BBa_C0079
BBa_K081004	.	tag	1543	1575	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K081004	.	sequence_alteration	1305	1305	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K081004	.	engineered_tag	1582	1606	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K081004	.	CDS	826	1542	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K081004	.	CDS	1740	2495	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K081004	.	point_mutation	2231	2231	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K081004	.	CDS	1740	2489	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K081004	.	engineered_region	1740	2495	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K081004	.	engineered_tag	2496	2520	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K081004	.	point_mutation	1913	1913	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K081004	.	engineered_region	1740	1741	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K081004	.	promoter	2576	2630	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K081004	.	promoter	2617	2622	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K081004	.	engineered_region	2576	2630	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K081004	.	promoter	2595	2600	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K081004	.	start_codon	2628	2628	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K081004	.	non_covalent_binding_site	2576	2595	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K081004	.	terminator	1615	1653	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K081004	.	polyA_site	1646	1653	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K081004	.	engineered_region	1615	1653	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K081004	.	stem_loop	1624	1645	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K081004	.	polyA_site	1615	1623	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K081004	.	CDS	22	796	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K081004	.	tag	733	765	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K081004	.	CDS	25	732	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K081004	.	engineered_tag	772	796	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
>BBa_K081004
attaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgcctta
aagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtc
aggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagtt
agcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcactta
gaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaagg
tgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatg
accgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccag
gtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgttttt
acaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcag
tggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgta
gatcgctactagagattaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtgg
aaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcct
aaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctg
gctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctacca
gacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggt
gctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcgg
tcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaa
accggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggtt
atctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcg
tagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaata
acactgatagtgctagtgtagatcactactagagaaaaaaaaaccccgcccctgacagggcggggtttttttttactag
agtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagattaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
aaaaaaaaaccccgcccctgacagggcggggtttttttttactagagacctgtaggatcgtacaggtttacgcaagaaa
atggtttgttatagtcgaataaa