LOCUS       BBa_K082021             1004 bp    DNA     linear       23-Dec-2024
DEFINITION  RhlI generator
ACCESSION   BBa_K082021
VERSION     BBa_K082021.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..803
                     /label=rhlR
     misc_feature    804..836
                     /label=LVA
     misc_feature    843..867
                     /label=Help:Barcodes
     CDS             81..842
                     /label=BBa_C0071
     stem_loop       887..930
                     /label=stem_loop
     misc_feature    876..955
                     /label=BBa_B0010
     terminator      876..955
                     /label=BBa_B0010
     stem_loop       971..990
                     /label=T7 TE
     misc_feature    997..997
                     /label=stop
     polyA_site      991..1004
                     /label=polya
     misc_feature    964..1004
                     /label=BBa_B0012
     terminator      964..1004
                     /label=BBa_B0012
     misc_feature    63..1004
                     /label=BBa_I0466
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaggaatg acggaggctt tttgctgtgg tgggacggtt
      121 tgcgtagcga gatgcagccg atccacgaca gccagggcgt gttcgccgtc ctggaaaagg
      181 aagtgcggcg cctgggcttc gattactacg cctatggcgt gcgccacacg attcccttca
      241 cccggccgaa gaccgaggtc catggcacct atcccaaggc ctggctggag cgataccaga
      301 tgcagaacta cggggccgtg gatccggcga tcctcaacgg cctgcgctcc tcggaaatgg
      361 tggtctggag cgacagcctg ttcgaccaga gccggatgct ctggaacgag gctcgcgatt
      421 ggggcctctg tgtcggcgcg accttgccga tccgcgcgcc gaacaatttg ctcagcgtgc
      481 tttccgtggc gcgcgaccag cagaacatct ccagcttcga gcgcgaggaa atccgcctgc
      541 ggctgcgttg catgatcgag ttgctgaccc agaagctgac cgacctggag catccgatgc
      601 tgatgtccaa cccggtctgc ctgagccatc gcgaacgcga gatcctgcaa tggaccgccg
      661 acggcaagag ttccggggaa atcgccatca tcctgagcat ctccgagagc acggtgaact
      721 tccaccacaa gaacatccag aagaagttcg acgcgccgaa caagacgctg gctgccgcct
      781 acgccgcggc gctgggtctc atcgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      841 aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa aacgaaaggc
      901 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
      961 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//