##gff-version 3
##sequence-region BBa_K082021 1 1004
BBa_K082021	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K082021	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K082021	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K082021	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K082021	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K082021	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K082021	.	engineered_region	63	1004	.	+	0	ID=BBa_I0466;Name=BBa_I0466
BBa_K082021	.	terminator	876	955	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0466
BBa_K082021	.	stem_loop	887	930	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K082021	.	engineered_region	876	955	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K082021	.	terminator	964	1004	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0466
BBa_K082021	.	polyA_site	991	1004	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K082021	.	stem_loop	971	990	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K082021	.	engineered_region	964	1004	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K082021	.	stop_codon	997	997	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K082021	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0466
BBa_K082021	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K082021	.	CDS	81	842	.	+	0	ID=BBa_C0071;Name=BBa_C0071;Parent=BBa_I0466
BBa_K082021	.	CDS	81	803	.	+	0	ID=annotation301192;Name=rhlR;Parent=BBa_C0071
BBa_K082021	.	tag	804	836	.	+	0	ID=annotation306523;Name=LVA;Parent=BBa_C0071
BBa_K082021	.	engineered_tag	843	867	.	+	0	ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071
>BBa_K082021
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaggaatgacggaggctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggc
gtgttcgccgtcctggaaaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattccct
tcacccggccgaagaccgaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggc
cgtggatccggcgatcctcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccgg
atgctctggaacgaggctcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctca
gcgtgctttccgtggcgcgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcat
gatcgagttgctgacccagaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgc
gaacgcgagatcctgcaatggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagca
cggtgaacttccaccacaagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggc
gctgggtctcatcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcacta
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata