LOCUS BBa_K082028 1165 bp DNA linear 26-Dec-2024 DEFINITION R0040-B0034-LacI ACCESSION BBa_K082028 VERSION BBa_K082028.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 83..1165 /label=BBa_K082004 CDS 83..1165 /label=BBa_K082004 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag aggtgaaacc agtaacgtta tacgatgtcg cagagtatgc 121 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa 181 aacgcgggaa gaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc 241 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct 301 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag 361 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa 421 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc 481 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca 541 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca 601 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc 661 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat 721 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct 781 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc 841 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata 901 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt 961 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt 1021 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa 1081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 1141 ttcccgactg gaaagcgggc agtga //