LOCUS       BBa_K082036             1890 bp    DNA     linear       22-Nov-2024
DEFINITION  LuxR & RhlI generator
ACCESSION   BBa_K082036
VERSION     BBa_K082036.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     misc_feature    63..998
                     /label=BBa_I0462
     misc_feature    1007..1025
                     /label=TetR 1
     misc_feature    1007..1060
                     /label=BBa_R0040
     promoter        1026..1031
                     /label=-35
     misc_feature    1032..1050
                     /label=TetR 2
     promoter        1049..1054
                     /label=-10
     promoter        1007..1060
                     /label=BBa_R0040
     misc_feature    1073..1076
                     /label=conserved
     RBS             1069..1080
                     /label=BBa_B0034
     CDS             1087..1689
                     /label=rhlL
     misc_feature    1690..1722
                     /label=LVA
     misc_feature    1729..1753
                     /label=Help:Barcodes
     CDS             1087..1753
                     /label=BBa_C0070
     stem_loop       1773..1816
                     /label=stem_loop
     misc_feature    1762..1841
                     /label=BBa_B0010
     terminator      1762..1841
                     /label=BBa_B0010
     stem_loop       1857..1876
                     /label=T7 TE
     misc_feature    1883..1883
                     /label=stop
     polyA_site      1877..1890
                     /label=polya
     misc_feature    1850..1890
                     /label=BBa_B0012
     terminator      1850..1890
                     /label=BBa_B0012
     terminator      1762..1890
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc tatcagtgat
     1021 agagattgac atccctatca gtgatagaga tactgagcac tactagagaa agaggagaaa
     1081 tactagatga tcgaactgct gtccgaatcc ctggaaggtc tgtccgctgc tatgatcgct
     1141 gaactgggtc gttaccgtca ccaggttttc atcgaaaaac tgggttggga cgttgtttcc
     1201 acctcccgtg ttcgtgacca ggagttcgac cagttcgacc acccgcagac ccgttacatc
     1261 gttgctatgt cccgtcaggg tatctgcggt tgcgctcgtc tgctgccgac caccgacgct
     1321 tacctgctga aagacgtttt cgcttacctg tgctccgaaa ccccgccgtc cgacccgtcc
     1381 gtttgggaac tgtcccgtta cgctgcttcc gctgctgacg acccgcagct ggctatgaaa
     1441 atcttctggt cctccctcca gtgcgcttgg tacctgggtg cttcctccgt tgttgctgtt
     1501 accaccaccg ctatggaacg ttacttcgtt cgtaacggtg ttatcctcca gcgtctgggt
     1561 ccgccgcaga aagttaaagg tgaaaccctg gttgctatct ccttcccggc ttaccaggaa
     1621 cgtggtctgg aaatgctgct gcgttaccac ccggaatggc tccagggtgt tccgctgtcc
     1681 atggctgttg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc
     1741 tagtgtagat ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     1801 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
     1861 caccttcggg tgggcctttc tgcgtttata
//