LOCUS       BBa_K082037             1633 bp    DNA     linear       22-Dec-2024
DEFINITION  RhlI & GFP generator
ACCESSION   BBa_K082037
VERSION     BBa_K082037.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..683
                     /label=rhlL
     misc_feature    684..716
                     /label=LVA
     misc_feature    723..747
                     /label=Help:Barcodes
     CDS             81..747
                     /label=BBa_C0070
     RBS             756..770
                     /label=RBS-1\Strong
     RBS             763..767
                     /label=RBS
     misc_feature    756..770
                     /label=BBa_B0030
     RBS             756..770
                     /label=BBa_B0030
     CDS             777..1496
                     /label=GFP protein
     CDS             777..1496
                     /label=BBa_E0040
     stem_loop       1516..1559
                     /label=stem_loop
     misc_feature    1505..1584
                     /label=BBa_B0010
     terminator      1505..1584
                     /label=BBa_B0010
     stem_loop       1600..1619
                     /label=T7 TE
     misc_feature    1626..1626
                     /label=stop
     polyA_site      1620..1633
                     /label=polya
     misc_feature    1593..1633
                     /label=BBa_B0012
     terminator      1593..1633
                     /label=BBa_B0012
     misc_feature    756..1633
                     /label=BBa_E0840
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatcgaac tgctgtccga atccctggaa ggtctgtccg
      121 ctgctatgat cgctgaactg ggtcgttacc gtcaccaggt tttcatcgaa aaactgggtt
      181 gggacgttgt ttccacctcc cgtgttcgtg accaggagtt cgaccagttc gaccacccgc
      241 agacccgtta catcgttgct atgtcccgtc agggtatctg cggttgcgct cgtctgctgc
      301 cgaccaccga cgcttacctg ctgaaagacg ttttcgctta cctgtgctcc gaaaccccgc
      361 cgtccgaccc gtccgtttgg gaactgtccc gttacgctgc ttccgctgct gacgacccgc
      421 agctggctat gaaaatcttc tggtcctccc tccagtgcgc ttggtacctg ggtgcttcct
      481 ccgttgttgc tgttaccacc accgctatgg aacgttactt cgttcgtaac ggtgttatcc
      541 tccagcgtct gggtccgccg cagaaagtta aaggtgaaac cctggttgct atctccttcc
      601 cggcttacca ggaacgtggt ctggaaatgc tgctgcgtta ccacccggaa tggctccagg
      661 gtgttccgct gtccatggct gttgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      721 aactctgata gtgctagtgt agatctctac tagagattaa agaggagaaa tactagatgc
      781 gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg
      841 ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac
      901 ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca acacttgtca
      961 ctactttcgg ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg
     1021 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
     1081 atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata
     1141 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg
     1201 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca
     1261 aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt
     1321 atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt
     1381 ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg
     1441 agtttgtaac agctgctggg attacacatg gcatggatga actatacaaa taataatact
     1501 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1561 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1621 ttctgcgttt ata
//