##gff-version 3
##sequence-region BBa_K082037 1 1633
BBa_K082037	.	engineered_region	756	1633	.	+	0	ID=BBa_E0840;Name=BBa_E0840
BBa_K082037	.	ribosome_entry_site	756	770	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_E0840
BBa_K082037	.	engineered_region	756	770	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K082037	.	ribosome_entry_site	763	767	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K082037	.	ribosome_entry_site	756	770	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K082037	.	CDS	777	1496	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0840
BBa_K082037	.	CDS	777	1496	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K082037	.	terminator	1593	1633	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0840
BBa_K082037	.	engineered_region	1593	1633	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K082037	.	stop_codon	1626	1626	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K082037	.	stem_loop	1600	1619	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K082037	.	polyA_site	1620	1633	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K082037	.	terminator	1505	1584	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0840
BBa_K082037	.	engineered_region	1505	1584	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K082037	.	stem_loop	1516	1559	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K082037	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K082037	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K082037	.	CDS	81	747	.	+	0	ID=BBa_C0070;Name=BBa_C0070
BBa_K082037	.	tag	684	716	.	+	0	ID=annotation306820;Name=LVA;Parent=BBa_C0070
BBa_K082037	.	engineered_tag	723	747	.	+	0	ID=annotation2213996;Name=Help:Barcodes;Parent=BBa_C0070
BBa_K082037	.	CDS	81	683	.	+	0	ID=annotation301203;Name=rhlL;Parent=BBa_C0070
BBa_K082037	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K082037	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K082037	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K082037	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K082037	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K082037	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
>BBa_K082037
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgatcgaactgctgtccgaatccctggaaggtctgtccgctgctatgatcgctgaactgggtcgttaccgtcaccag
gttttcatcgaaaaactgggttgggacgttgtttccacctcccgtgttcgtgaccaggagttcgaccagttcgaccacc
cgcagacccgttacatcgttgctatgtcccgtcagggtatctgcggttgcgctcgtctgctgccgaccaccgacgctta
cctgctgaaagacgttttcgcttacctgtgctccgaaaccccgccgtccgacccgtccgtttgggaactgtcccgttac
gctgcttccgctgctgacgacccgcagctggctatgaaaatcttctggtcctccctccagtgcgcttggtacctgggtg
cttcctccgttgttgctgttaccaccaccgctatggaacgttacttcgttcgtaacggtgttatcctccagcgtctggg
tccgccgcagaaagttaaaggtgaaaccctggttgctatctccttcccggcttaccaggaacgtggtctggaaatgctg
ctgcgttaccacccggaatggctccagggtgttccgctgtccatggctgttgctgcaaacgacgaaaactacgctttag
tagcttaataactctgatagtgctagtgtagatctctactagagattaaagaggagaaatactagatgcgtaaaggaga
agaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtgga
gagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggc
caacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgacttttt
caagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgct
gaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattc
ttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagt
taacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggc
gatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagag
accacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata