LOCUS       BBa_K085004             1345 bp    DNA     linear       22-Dec-2024
DEFINITION  riboswitch system with GFP
ACCESSION   BBa_K085004
VERSION     BBa_K085004.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    209..302
                     /label=BBa_J23008
     terminator      311..405
                     /label=BBa_B0024
     misc_feature    414..432
                     /label=TetR 1
     misc_feature    414..467
                     /label=BBa_R0040
     promoter        433..438
                     /label=-35
     misc_feature    439..457
                     /label=TetR 2
     promoter        456..461
                     /label=-10
     promoter        414..467
                     /label=BBa_R0040
     misc_feature    476..518
                     /label=BBa_J23032
     misc_feature    525..1242
                     /label=BBa_K085001
     terminator      1251..1345
                     /label=BBa_B0024
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagac ccaaaagcaa gaggtgattc tagttggtgg
      241 ttaatgaaaa ttaacttact tactagaaat atctctaaaa agccagatta ttaatccggc
      301 tttactagag aaataataaa aaagccggat taataatctg gctttttata ttctctctct
      361 agtatataaa cgcagaaagg cccacccgaa ggtgagccag tgtgatacta gagtccctat
      421 cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac tagagaacta
      481 gaatcacctc ttgcttttgg gtaagacaga agaggagata ctagatggct agcaaaggag
      541 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      601 acaaattttc tgtcagtgga gagggtgaag gtgatgctac atacggaaag cttaccctta
      661 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttct
      721 cttatggtgt tcaatgcttt tcccgttatc cggatcatat gaaacggcat gactttttca
      781 agagtgccat gcccgaaggt tatgtacagg aacgcactat atctttcaaa gatgacggga
      841 actacaagac gcgtgctgaa gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt
      901 taaaaggtat tgattttaaa gaagatggaa acattctcgg acacaaactc gagtacaact
      961 ataactcaca caatgtatac atcacggcag acaaacaaaa gaatggaatc aaagctaact
     1021 tcaaaattcg ccacaacatt gaagatggat ccgttcaact agcagaccat tatcaacaaa
     1081 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tcgacacaat
     1141 ctgccctttc gaaagatccc aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa
     1201 ctgctgctgg gattacacat ggcatggatg agctctacaa attactagag aaataataaa
     1261 aaagccggat taataatctg gctttttata ttctctctct agtatataaa cgcagaaagg
     1321 cccacccgaa ggtgagccag tgtga
//