LOCUS BBa_K086014 941 bp DNA linear 23-Dec-2024 DEFINITION modified Lutz-Bujard LacO promoter,with alternative sigma factor σ38 followed by YFP reporter ACCESSION BBa_K086014 VERSION BBa_K086014.1 FEATURES Location/Qualifiers promoter 1..55 /label=P38_2 promoter 1..55 /label=BBa_K086031 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 82..804 /label=BBa_E0030 stem_loop 824..867 /label=stem_loop misc_feature 813..892 /label=BBa_B0010 terminator 813..892 /label=BBa_B0010 stem_loop 908..927 /label=T7 TE misc_feature 934..934 /label=stop polyA_site 928..941 /label=polya misc_feature 901..941 /label=BBa_B0012 terminator 901..941 /label=BBa_B0012 ORIGIN 1 aattgtgagc ggataacaat tctcacagtg agcgagtaac aactacgctg ttttatacta 61 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 241 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc 301 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac 361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 661 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac 721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 781 atggacgagc tgtacaagta ataatactag agccaggcat caaataaaac gaaaggctca 841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 901 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //