LOCUS       BBa_K088001             1378 bp    DNA     linear       22-Dec-2024
DEFINITION  RBS+lacI+pLac
ACCESSION   BBa_K088001
VERSION     BBa_K088001.1  
FEATURES             Location/Qualifiers
     RBS             1..11
                     /label=RBS-4\Weaker
     RBS             7..10
                     /label=RBS
     misc_feature    1..11
                     /label=BBa_B0033
     RBS             1..11
                     /label=BBa_B0033
     misc_feature    1107..1145
                     /label=LVA
     CDS             18..1145
                     /label=lacI-LVA
     misc_feature    18..1145
                     /label=BBa_C0012
     misc_feature    1146..1170
                     /label=Help:Barcodes
     CDS             18..1145
                     /label=BBa_C0012
     CDS             1179..1266
                     /label=end of LacI coding region (inactive)
     misc_feature    1179..1378
                     /label=BBa_R0010
     misc_feature    1267..1304
                     /label=CAP binding site
     promoter        1315..1320
                     /label=-35
     promoter        1339..1344
                     /label=-10
     misc_feature    1344..1378
                     /label=LacI binding site
     misc_feature    1351..1351
                     /label=start
     promoter        1179..1378
                     /label=BBa_R0010
ORIGIN
        1 tcacacagga ctactagatg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt
       61 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
      121 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
      181 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg
      241 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg
      301 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc
      361 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg
      421 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg
      481 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg
      541 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg
      601 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc
      661 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa
      721 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg
      781 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
      841 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg
      901 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
      961 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc
     1021 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     1081 aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct ttagtagctt
     1141 aataactctg atagtgctag tgtagatctc tactagagca atacgcaaac cgcctctccc
     1201 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     1261 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     1321 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacaca
//