LOCUS       BBa_K088002             1378 bp    DNA     linear       21-May-2024
DEFINITION  pLac+RBS+lacI
ACCESSION   BBa_K088002
VERSION     BBa_K088002.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..219
                     /label=RBS-4\Weaker
     RBS             215..218
                     /label=RBS
     misc_feature    209..219
                     /label=BBa_B0033
     RBS             209..219
                     /label=BBa_B0033
     misc_feature    1315..1353
                     /label=LVA
     CDS             226..1353
                     /label=lacI-LVA
     misc_feature    226..1353
                     /label=BBa_C0012
     misc_feature    1354..1378
                     /label=Help:Barcodes
     CDS             226..1353
                     /label=BBa_C0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggact actagatggt gaatgtgaaa
      241 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
      301 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
      361 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
      421 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
      481 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
      541 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
      601 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
      661 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
      721 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
      781 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
      841 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
      901 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
      961 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
     1021 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat
     1081 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
     1141 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
     1201 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     1261 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcaggctgca
     1321 aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg tagatctc
//