LOCUS BBa_K088003 3357 bp DNA linear 25-Nov-2024 DEFINITION RBS+lacI+pLac+ RBS+cI ACCESSION BBa_K088003 VERSION BBa_K088003.1 FEATURES Location/Qualifiers RBS 1..11 /label=RBS-4\Weaker RBS 7..10 /label=RBS misc_feature 1..11 /label=BBa_B0033 RBS 1..11 /label=BBa_B0033 misc_feature 1107..1145 /label=LVA CDS 18..1145 /label=lacI-LVA misc_feature 18..1145 /label=BBa_C0012 misc_feature 1146..1170 /label=Help:Barcodes CDS 18..1145 /label=BBa_C0012 CDS 1179..1266 /label=end of LacI coding region (inactive) misc_feature 1179..1378 /label=BBa_R0010 misc_feature 1267..1304 /label=CAP binding site promoter 1315..1320 /label=-35 promoter 1339..1344 /label=-10 misc_feature 1344..1378 /label=LacI binding site misc_feature 1351..1351 /label=start promoter 1179..1378 /label=BBa_R0010 RBS 1387..1397 /label=RBS-4\Weaker RBS 1393..1396 /label=RBS misc_feature 1387..1397 /label=BBa_B0033 RBS 1387..1397 /label=BBa_B0033 misc_feature 2493..2531 /label=LVA CDS 1404..2531 /label=lacI-LVA misc_feature 1404..2531 /label=BBa_C0012 misc_feature 2532..2556 /label=Help:Barcodes CDS 1404..2531 /label=BBa_C0012 misc_feature 2569..2572 /label=conserved RBS 2565..2576 /label=BBa_B0034 CDS 2586..3293 /label=cI lambda misc_feature 3294..3326 /label=LVA misc_feature 3333..3357 /label=Help:Barcodes CDS 2583..3357 /label=BBa_C0051 ORIGIN 1 tcacacagga ctactagatg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt 61 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg 121 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg 181 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg 241 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg 301 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc 361 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg 421 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg 481 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg 541 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg 601 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc 661 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa 721 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg 781 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg 841 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg 901 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg 961 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc 1021 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 1081 aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct ttagtagctt 1141 aataactctg atagtgctag tgtagatctc tactagagca atacgcaaac cgcctctccc 1201 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 1261 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 1321 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacata 1381 ctagagtcac acaggactac tagatggtga atgtgaaacc agtaacgtta tacgatgtcg 1441 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg 1501 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca 1561 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca 1621 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac 1681 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg 1741 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg 1801 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg 1861 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg 1921 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa 1981 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa 2041 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca 2101 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg 2161 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg 2221 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca 2281 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg 2341 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc 2401 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg 2461 cacgacaggt ttcccgactg gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag 2521 tagcttaata actctgatag tgctagtgta gatctctact agagaaagag gagaaatact 2581 agatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca cgtcgcctta 2641 aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca 2701 agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat gcattaaatg 2761 cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa tttagccctt 2821 caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg tcacttagaa 2881 gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca cctgagctta 2941 gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa gccagtgatt 3001 ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc tccaagccaa 3061 gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag ccaggtgatt 3121 tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc agggatagcg 3181 gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc aatgagagtt 3241 gttccgttgt ggggaaagtt atcgctagtc agtggcctga agagacgttt ggcgctgcaa 3301 acgacgaaaa ctacgcttta gtagcttaat aacgctgata gtgctagtgt agatcgc //