##gff-version 3
##sequence-region BBa_K088003 1 3357
BBa_K088003	.	ribosome_entry_site	1	11	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K088003	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K088003	.	ribosome_entry_site	1	11	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K088003	.	engineered_region	1	11	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K088003	.	CDS	2583	3357	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K088003	.	tag	3294	3326	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K088003	.	CDS	2586	3293	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K088003	.	engineered_tag	3333	3357	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K088003	.	promoter	1179	1378	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K088003	.	non_covalent_binding_site	1344	1378	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K088003	.	non_covalent_binding_site	1267	1304	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K088003	.	engineered_region	1179	1378	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K088003	.	promoter	1315	1320	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K088003	.	CDS	1179	1266	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K088003	.	start_codon	1351	1351	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K088003	.	promoter	1339	1344	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K088003	.	ribosome_entry_site	2565	2576	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K088003	.	conserved_region	2569	2572	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K088003	.	CDS	18	1145	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K088003	.	engineered_tag	1146	1170	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K088003	.	CDS	18	1145	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K088003	.	tag	1107	1145	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K088003	.	engineered_region	18	1145	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K088003	.	ribosome_entry_site	1387	1397	.	+	0	ID=BBa_B0033;Name=BBa_B0033
BBa_K088003	.	ribosome_entry_site	1393	1396	.	+	0	ID=annotation1714;Name=RBS;Parent=BBa_B0033
BBa_K088003	.	ribosome_entry_site	1387	1397	.	+	0	ID=annotation1713;Name=RBS-4\Weaker;Parent=BBa_B0033
BBa_K088003	.	engineered_region	1387	1397	.	+	0	ID=annotation7028;Name=BBa_B0033;Parent=BBa_B0033
BBa_K088003	.	CDS	1404	2531	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_K088003	.	engineered_tag	2532	2556	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K088003	.	CDS	1404	2531	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K088003	.	tag	2493	2531	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K088003	.	engineered_region	1404	2531	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
>BBa_K088003
tcacacaggactactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatca
gaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggag
ctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtc
tggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtc
gatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatc
attaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatg
tctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgc
attgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcat
aaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaa
ccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcg
cgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgt
tatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactct
ctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatac
gcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcag
gctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagcaatacg
caaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagt
gagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgt
tgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggactactagatggtgaatgtgaaaccag
taacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgt
ttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactg
gcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcga
ttaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagc
ggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgct
gtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttct
cccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggccc
attaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcg
gaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactg
cgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcgga
tatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggatttt
cgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgc
ccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcatt
aatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaa
taactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaa
cacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccagga
atctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttat
aacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgaga
tgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggat
gttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattct
gcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaa
ttctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaa
gaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagt
tgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctt
tagtagcttaataacgctgatagtgctagtgtagatcgc