LOCUS       BBa_K088004             1802 bp    DNA     linear       22-May-2024
DEFINITION  RBS+GFP+Pr+ RBS+mRFP
ACCESSION   BBa_K088004
VERSION     BBa_K088004.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..739
                     /label=GFP protein
     CDS             20..739
                     /label=BBa_E0040
     stem_loop       759..802
                     /label=stem_loop
     misc_feature    748..827
                     /label=BBa_B0010
     terminator      748..827
                     /label=BBa_B0010
     stem_loop       843..862
                     /label=T7 TE
     misc_feature    869..869
                     /label=stop
     polyA_site      863..876
                     /label=polya
     misc_feature    836..876
                     /label=BBa_B0012
     terminator      836..876
                     /label=BBa_B0012
     promoter        922..927
                     /label=-10
     promoter        899..904
                     /label=-35
     misc_feature    909..925
                     /label=OR1
     misc_feature    885..901
                     /label=OR2
     misc_feature    885..933
                     /label=BBa_R0051
     promoter        885..933
                     /label=BBa_R0051
     misc_feature    946..949
                     /label=conserved
     RBS             942..953
                     /label=BBa_B0034
     CDS             960..1634
                     /label=mrfp1
     misc_feature    1641..1665
                     /label=Help:Barcodes
     CDS             960..1665
                     /label=BBa_E1010
     stem_loop       1685..1728
                     /label=stem_loop
     misc_feature    1674..1753
                     /label=BBa_B0010
     terminator      1674..1753
                     /label=BBa_B0010
     stem_loop       1769..1788
                     /label=T7 TE
     misc_feature    1795..1795
                     /label=stop
     polyA_site      1789..1802
                     /label=polya
     misc_feature    1762..1802
                     /label=BBa_B0012
     terminator      1762..1802
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
       61 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      121 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      181 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      241 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      301 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      361 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      421 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      481 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      541 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      601 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      661 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      721 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      781 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      841 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtaacac cgtgcgtgtt
      901 gactatttta cctctggcgg tgataatggt tgctactaga gaaagaggag aaatactaga
      961 tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt
     1021 ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta
     1081 cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc
     1141 tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg
     1201 actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag
     1261 acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca
     1321 aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca
     1381 tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa
     1441 tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct
     1501 acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca
     1561 tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc
     1621 actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca
     1681 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     1741 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     1801 ta
//