LOCUS       BBa_K088005             2811 bp    DNA     linear       21-May-2024
DEFINITION  pL+lacI+cI+GFP+Pr+mRFP
ACCESSION   BBa_K088005
VERSION     BBa_K088005.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             230..937
                     /label=cI lambda
     misc_feature    938..970
                     /label=LVA
     misc_feature    977..1001
                     /label=Help:Barcodes
     CDS             227..976
                     /label=BBa_C0051
     RBS             1010..1022
                     /label=RBS-3\Weak
     RBS             1016..1019
                     /label=RBS
     misc_feature    1010..1022
                     /label=BBa_B0032
     RBS             1010..1022
                     /label=BBa_B0032
     CDS             1029..1748
                     /label=GFP protein
     CDS             1029..1748
                     /label=BBa_E0040
     stem_loop       1768..1811
                     /label=stem_loop
     misc_feature    1757..1836
                     /label=BBa_B0010
     terminator      1757..1836
                     /label=BBa_B0010
     stem_loop       1852..1871
                     /label=T7 TE
     misc_feature    1878..1878
                     /label=stop
     polyA_site      1872..1885
                     /label=polya
     misc_feature    1845..1885
                     /label=BBa_B0012
     terminator      1845..1885
                     /label=BBa_B0012
     promoter        1931..1936
                     /label=-10
     promoter        1908..1913
                     /label=-35
     misc_feature    1918..1934
                     /label=OR1
     misc_feature    1894..1910
                     /label=OR2
     misc_feature    1894..1942
                     /label=BBa_R0051
     promoter        1894..1942
                     /label=BBa_R0051
     misc_feature    1955..1958
                     /label=conserved
     RBS             1951..1962
                     /label=BBa_B0034
     CDS             1969..2643
                     /label=mrfp1
     misc_feature    2650..2674
                     /label=Help:Barcodes
     CDS             1969..2649
                     /label=BBa_E1010
     stem_loop       2694..2737
                     /label=stem_loop
     misc_feature    2683..2762
                     /label=BBa_B0010
     terminator      2683..2762
                     /label=BBa_B0010
     stem_loop       2778..2797
                     /label=T7 TE
     misc_feature    2804..2804
                     /label=stop
     polyA_site      2798..2811
                     /label=polya
     misc_feature    2771..2811
                     /label=BBa_B0012
     terminator      2771..2811
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gcacaaaaaa
      241 gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa
      301 aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca
      361 gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt
      421 gcttgcaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat
      481 ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc
      541 tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg
      601 tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga
      661 ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat
      721 gttaattctc gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact
      781 tgggggtgat gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca
      841 accactaaac ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa
      901 agttatcgct agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc
      961 tttagtagct taataacgct gatagtgcta gtgtagatcg ctactagagt cacacaggaa
     1021 agtactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     1081 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     1141 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
     1201 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata
     1261 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
     1321 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
     1381 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
     1441 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa
     1501 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac
     1561 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     1621 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     1681 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
     1741 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1801 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1861 tcgggtgggc ctttctgcgt ttatatacta gagtaacacc gtgcgtgttg actattttac
     1921 ctctggcggt gataatggtt gctactagag aaagaggaga aatactagat ggcttcctcc
     1981 gaagacgtta tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt
     2041 cacgagttcg aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct
     2101 aaactgaaag ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag
     2161 ttccagtacg gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa
     2221 ctgtccttcc cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt
     2281 gttaccgtta cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg
     2341 cgtggtacca acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa
     2401 gcttccaccg aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt
     2461 ctgaaactga aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa
     2521 aaaccggttc agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac
     2581 aacgaagact acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt
     2641 gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac
     2701 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2761 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//