LOCUS BBa_K088006 3989 bp DNA linear 21-May-2024 DEFINITION lacI+pL+cI+GFP+Pr+mRFP ACCESSION BBa_K088006 VERSION BBa_K088006.1 FEATURES Location/Qualifiers RBS 1..11 /label=RBS-4\Weaker RBS 7..10 /label=RBS misc_feature 1..11 /label=BBa_B0033 RBS 1..11 /label=BBa_B0033 misc_feature 1107..1145 /label=LVA CDS 18..1145 /label=lacI-LVA misc_feature 18..1145 /label=BBa_C0012 misc_feature 1146..1170 /label=Help:Barcodes CDS 18..1145 /label=BBa_C0012 CDS 1179..1266 /label=end of LacI coding region (inactive) misc_feature 1179..1378 /label=BBa_R0010 misc_feature 1267..1304 /label=CAP binding site promoter 1315..1320 /label=-35 promoter 1339..1344 /label=-10 misc_feature 1344..1378 /label=LacI binding site misc_feature 1351..1351 /label=start promoter 1179..1378 /label=BBa_R0010 misc_feature 1391..1394 /label=conserved RBS 1387..1398 /label=BBa_B0034 CDS 1408..2115 /label=cI lambda misc_feature 2116..2148 /label=LVA misc_feature 2155..2179 /label=Help:Barcodes CDS 1405..2179 /label=BBa_C0051 RBS 2188..2200 /label=RBS-3\Weak RBS 2194..2197 /label=RBS misc_feature 2188..2200 /label=BBa_B0032 RBS 2188..2200 /label=BBa_B0032 CDS 2207..2926 /label=GFP protein CDS 2207..2926 /label=BBa_E0040 stem_loop 2946..2989 /label=stem_loop misc_feature 2935..3014 /label=BBa_B0010 terminator 2935..3014 /label=BBa_B0010 stem_loop 3030..3049 /label=T7 TE misc_feature 3056..3056 /label=stop polyA_site 3050..3063 /label=polya misc_feature 3023..3063 /label=BBa_B0012 terminator 3023..3063 /label=BBa_B0012 promoter 3109..3114 /label=-10 promoter 3086..3091 /label=-35 misc_feature 3096..3112 /label=OR1 misc_feature 3072..3088 /label=OR2 misc_feature 3072..3120 /label=BBa_R0051 promoter 3072..3120 /label=BBa_R0051 misc_feature 3133..3136 /label=conserved RBS 3129..3140 /label=BBa_B0034 CDS 3147..3821 /label=mrfp1 misc_feature 3828..3852 /label=Help:Barcodes CDS 3147..3852 /label=BBa_E1010 stem_loop 3872..3915 /label=stem_loop misc_feature 3861..3940 /label=BBa_B0010 terminator 3861..3940 /label=BBa_B0010 stem_loop 3956..3975 /label=T7 TE misc_feature 3982..3982 /label=stop polyA_site 3976..3989 /label=polya misc_feature 3949..3989 /label=BBa_B0012 terminator 3949..3989 /label=BBa_B0012 ORIGIN 1 tcacacagga ctactagatg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt 61 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg 121 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg 181 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg 241 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg 301 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc 361 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg 421 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg 481 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg 541 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg 601 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc 661 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa 721 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg 781 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg 841 gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg 901 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg 961 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc 1021 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 1081 aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct ttagtagctt 1141 aataactctg atagtgctag tgtagatctc tactagagca atacgcaaac cgcctctccc 1201 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg 1261 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 1321 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacata 1381 ctagagaaag aggagaaata ctagatgagc acaaaaaaga aaccattaac acaagagcag 1441 cttgaggacg cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga acttggctta 1501 tcccaggaat ctgtcgcaga caagatgggg atggggcagt caggcgttgg tgctttattt 1561 aatggcatca atgcattaaa tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt 1621 agcgttgaag aatttagccc ttcaatcgcc agagaaatct acgagatgta tgaagcggtt 1681 agtatgcagc cgtcacttag aagtgagtat gagtaccctg ttttttctca tgttcaggca 1741 gggatgttct cacctgagct tagaaccttt accaaaggtg atgcggagag atgggtaagc 1801 acaaccaaaa aagccagtga ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc 1861 gcaccaacag gctccaagcc aagctttcct gacggaatgt taattctcgt tgaccctgag 1921 caggctgttg agccaggtga tttctgcata gccagacttg ggggtgatga gtttaccttc 1981 aagaaactga tcagggatag cggtcaggtg tttttacaac cactaaaccc acagtaccca 2041 atgatcccat gcaatgagag ttgttccgtt gtggggaaag ttatcgctag tcagtggcct 2101 gaagagacgt ttggcgctgc aaacgacgaa aactacgctt tagtagctta ataacgctga 2161 tagtgctagt gtagatcgct actagagtca cacaggaaag tactagatgc gtaaaggaga 2221 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca 2281 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa 2341 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg 2401 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa 2461 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa 2521 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt 2581 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta 2641 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt 2701 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa 2761 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc 2821 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac 2881 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagccaggc 2941 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 3001 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 3061 atatactaga gtaacaccgt gcgtgttgac tattttacct ctggcggtga taatggttgc 3121 tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca 3181 tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg 3241 aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg 3301 gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt 3361 acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca 3421 aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct 3481 ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg 3541 acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc 3601 cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc 3661 actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg 3721 cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg 3781 aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct 3841 agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 3901 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 3961 accttcgggt gggcctttct gcgtttata //