BBa_K090002
1
BBa_K090002
Constitutively expresses glutamate gated K+ channel
2008-10-22T11:00:00Z
2015-05-08T01:08:37Z
From genome of Synechocystis PCC 6803.
Glutamate gated K+ channel BBa_K090000 (GluR0)- under control of constitutive promoter BBa_J23116 and RBS BBa_J61117. Contains terminator BBa_B1002
false
false
_188_
0
3483
9
Not in stock
false
From peptide sequence of Synechocystis PCC 6803, codon optimised for E.coli, restriction sites removed, and synthesised de novo. Promoter is constitutive, medium strength. RBS is medium strength. Terminator is very strong artificial.
false
Ellis O'Neill
component1985291
1
BBa_B1002
component1985285
1
BBa_J61117
component1985286
1
BBa_K090000
component1985284
1
BBa_J23116
annotation1985285
1
BBa_J61117
range1985285
1
44
55
annotation1985286
1
BBa_K090000
range1985286
1
62
1255
annotation1985284
1
BBa_J23116
range1985284
1
1
35
annotation1985291
1
BBa_B1002
range1985291
1
1264
1297
BBa_B1002
1
BBa_B1002
Terminator (artificial, small, %T~=85%)
2006-08-29T11:00:00Z
2015-08-31T04:07:21Z
antiquity
Artifical terminator, estimated %T~=85
false
true
_41_
0
745
41
In stock
false
Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues.
true
Haiyao Huang
annotation1898412
1
B1002
range1898412
1
1
34
annotation1898414
1
Poly A tail
range1898414
1
25
31
annotation1898415
1
Poly A tail
range1898415
1
4
9
annotation1898413
1
stem loop
range1898413
1
10
25
BBa_J61117
1
BBa_J61117
Ribosome Binding Site Family Member
2007-04-23T11:00:00Z
2015-08-31T01:59:49Z
N/A
{{JCA_Arkin_RBSFamily}}
false
false
_95_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_K090000
1
BBa_K090000
Glutamate gated K+ channel
2008-10-22T11:00:00Z
2015-05-08T01:08:37Z
From Genomic sequence
This part encodes the gene GluR0 from Synechocystis. It is a well characterised glutamate-gated K+ membrane channel, which has a considerable degree of structural and functional homology to rat neurone GluR2 AMPA receptors.
false
false
_188_
0
3483
9
It's complicated
false
From peptide sequence of Synechocystis PCC 6803, codon optimised for E.coli, restriction sites removed, and synthesised de novo.
false
Ellis O'Neill
BBa_J23116
1
BBa_J23116
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Later
false
false
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K090002_sequence
1
ttgacagctagctcagtcctagggactatgctagctactagagaaagacatgagttactagatgctgatcctgcgtcgtctgatcctggttgtttctctgtctttcgcaactattgcaattgacatcactacccgtccgggtctggaaccggttcagggtcagggtgtcgaaaccgtagatagccagacgctgaaagttggcgttgttggtaacccgccgttcgttttctatggtgaaggtaaaaacgcggcgttcactggtatctctctggacgtttggcgtgcggtagcagaatcccaaaaatggaactctgagtatgttcgtcagaacagcatctctgctggcatcacggcggtcgcagaaggtgaactggacatcctgattggcccaattagcgtgaccccggaacgcgctgctatcgaaggtattactttcacccagccgtacttttcttctggtattggtctgctgatcccgggcaaaccggtgtccctgtgggaacgtttctctccgttctttggcattgcagctctgtctagcgctggcgttctgactctgctgctgttcctggtaggtaacctgatttggctggcggaacaccgcaaaaacccagaacagttcagcccgcactaccctgaaggcgttcagaacggtatgtggttcgccctggtgaccctgaccaccgtgggctacggcgatcgtagcccgcgtactaagctgggccagctggtagcgggtgtttggatgctggtagcgctgctgtctttttctagcattaccgccggtctggcgagcgctttcagcaccgcactgtctgaagcgtctgctacgccactgttccgctccgtcggcgacctgaaaaacaaggaagtcgctgttgtgcgtgacaccaccgctgtagactgggctaatttctaccaggcggatgtacgtgaaactaacaacctgaccgctgcaatcaccctgctgcaaaagaagcaagtagaggccgtcatgttcgaccgtccggctctgatctattatactcgtcagaaccctaacctgaatctggaggtgactgaaatccgtgtttctctggaaccttacggcttcgttctgaaagaaaactctccgctgcaaaagactattaacgtggaaatgctgaacctgctgtattctcgcgtcattgctgaatttaccgaacgttggctgggtccaggcatcgaagagaaccaggatctgctgccacagaacattggcgaatccccgtcctaatactagagcgcaaaaaaccccgcttcggcggggttttttcgc
BBa_J23116_sequence
1
ttgacagctagctcagtcctagggactatgctagc
BBa_J61117_sequence
1
aaagacatgagt
BBa_B1002_sequence
1
cgcaaaaaaccccgcttcggcggggttttttcgc
BBa_K090000_sequence
1
atgctgatcctgcgtcgtctgatcctggttgtttctctgtctttcgcaactattgcaattgacatcactacccgtccgggtctggaaccggttcagggtcagggtgtcgaaaccgtagatagccagacgctgaaagttggcgttgttggtaacccgccgttcgttttctatggtgaaggtaaaaacgcggcgttcactggtatctctctggacgtttggcgtgcggtagcagaatcccaaaaatggaactctgagtatgttcgtcagaacagcatctctgctggcatcacggcggtcgcagaaggtgaactggacatcctgattggcccaattagcgtgaccccggaacgcgctgctatcgaaggtattactttcacccagccgtacttttcttctggtattggtctgctgatcccgggcaaaccggtgtccctgtgggaacgtttctctccgttctttggcattgcagctctgtctagcgctggcgttctgactctgctgctgttcctggtaggtaacctgatttggctggcggaacaccgcaaaaacccagaacagttcagcccgcactaccctgaaggcgttcagaacggtatgtggttcgccctggtgaccctgaccaccgtgggctacggcgatcgtagcccgcgtactaagctgggccagctggtagcgggtgtttggatgctggtagcgctgctgtctttttctagcattaccgccggtctggcgagcgctttcagcaccgcactgtctgaagcgtctgctacgccactgttccgctccgtcggcgacctgaaaaacaaggaagtcgctgttgtgcgtgacaccaccgctgtagactgggctaatttctaccaggcggatgtacgtgaaactaacaacctgaccgctgcaatcaccctgctgcaaaagaagcaagtagaggccgtcatgttcgaccgtccggctctgatctattatactcgtcagaaccctaacctgaatctggaggtgactgaaatccgtgtttctctggaaccttacggcttcgttctgaaagaaaactctccgctgcaaaagactattaacgtggaaatgctgaacctgctgtattctcgcgtcattgctgaatttaccgaacgttggctgggtccaggcatcgaagagaaccaggatctgctgccacagaacattggcgaatccccgtcctaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z