BBa_K090004 1 BBa_K090004 Expresses KDP Potassium ATPase in Stationary Phase 2008-10-23T11:00:00Z 2015-05-08T01:08:37Z BBa_J45992, BBa_B0030, BBa_K090003 and BBa_B1002 Stationary phase promoter OsmY J45992 linked to strong RBS B0030, causing expression of KDP Potassium ATPase. Also has strong artifical terminator B1002 false false _188_ 0 3483 9 Not in stock false Must not be expressed too strongly as this would lead to toxicity issues. Choice of RBS important. false Ellis O'Neill component1986246 1 BBa_K090003 component1986240 1 BBa_B0030 component1986251 1 BBa_B1002 component1986238 1 BBa_J45992 annotation1986246 1 BBa_K090003 range1986246 1 231 4614 annotation1986238 1 BBa_J45992 range1986238 1 1 199 annotation1986251 1 BBa_B1002 range1986251 1 4623 4656 annotation1986240 1 BBa_B0030 range1986240 1 208 222 BBa_K090003 1 BBa_K090003 KDP Potassium ATPase 2008-10-23T11:00:00Z 2015-05-08T01:08:37Z From genome of E.coli. The KDP operon from E.coli, without the regulatory subunits. Pumps K+ ions into cells, against their concentration gradient, using ATP. false false _188_ 0 3483 9 Not in stock false Primers designed to only PCR A,B,C and F subunits, removing D and E regulatory subunits. false Ellis O'Neill annotation1986218 1 KDP A range1986218 1 90 1763 annotation1986219 1 KDP B range1986219 1 1764 3812 annotation1986216 1 KDP F range1986216 1 1 89 annotation1986217 1 KDP C range1986217 1 3813 4384 BBa_J45992 1 p(osmY) Full-length stationary phase osmY promoter 2006-07-10T11:00:00Z 2015-08-31T04:08:50Z This part was PCRed out of E. coli's genome. osmY's promoter is active in stationary phase and under high osmotic pressure conditions. false false _84_ 0 642 84 Not in stock true Truncated Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GGCT TAT GTT TTC GCT GAT ATC - 3' Total Length: 49 bp Annealing Length: 21 bp GC Content: 38.1% Melting Temperature: 49.5 degrees C hairpin deltaG: -2.66 kcal/mol self dimer deltaG: -99.97 kcal/mol Full Forward Primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GCTG GCA CAG GAA CGT TAT C - 3' Total Length: 47 bp Annealing Length: 19 bp GC Content: 52.6% Melting Temperature: 53.4 degrees C hairpin deltaG: -3.99 kcal/mol self dimer deltaG: -98.3 kcal/mol Reverse Primer: 5'-GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TGT TAA ATA TAG ATC ACA ATT TTG- 3' Total Length: 54 bp Annealing Length: 25 bp GC Content: 20.0% Melting Temperature: 46.4 degrees C hairpin deltaG: -2.86 kcal/mol self dimer deltaG: -99.44 kcal/mol heterodimer deltaG with Forward Primer: -99.44 kcal/mol heterodimer deltaG with Conserved Forward Primer: -99.97 kcal/mol false Stephen Payne annotation1883645 1 -10 range1883645 1 188 193 annotation1883646 1 -35 range1883646 1 164 169 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1701 1 RBS-1\Strong range1701 1 1 15 annotation1702 1 RBS range1702 1 8 12 BBa_B1002 1 BBa_B1002 Terminator (artificial, small, %T~=85%) 2006-08-29T11:00:00Z 2015-08-31T04:07:21Z antiquity Artifical terminator, estimated %T~=85 false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 6 residues. true Haiyao Huang annotation1898412 1 B1002 range1898412 1 1 34 annotation1898414 1 Poly A tail range1898414 1 25 31 annotation1898415 1 Poly A tail range1898415 1 4 9 annotation1898413 1 stem loop range1898413 1 10 25 BBa_B1002_sequence 1 cgcaaaaaaccccgcttcggcggggttttttcgc BBa_J45992_sequence 1 ctggcacaggaacgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtggcgccctgtcaatttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaa BBa_K090004_sequence 1 ctggcacaggaacgttatccggacgttcagttccaccagacccgcgagcattaattcttgcctccagggcgcggtagtggcgccctgtcaatttcccttccttattagccgcttacggaatgttcttaaaacattcacttttgcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaatactagagattaaagaggagaaatactagagtgagtgcaggcgtgataaccggcgtattgctggtgtttttattactgggttatctggtttatgccctgatcaatgcggaggcgttctgaatggctgcgcaagggttcttactgatcgccacgtttttactggtgttaatggtgctggcgcgtcctttaggcagcgggctggcgcggctgattaatgacattcctcttcccggtacaacgggcgttgagcgcgtactttttcgcgcacttggcgtctctgaccgtgagatgaactggaagcaatatctttgtgccattctcggcctgaacatgctggggctggcggtgctgttttttatgttgctcggtcagcactatctgccgcttaatccacagcagttgccagggctgtcgtgggatctggcgctgaataccgccgtcagctttgtcaccaataccaactggcaatcttatagcggtgaaaccacgttgagctatttcagccagatggcgggcttaacggtgcaaaactttctttctgccgccagcgggattgcggtgatttttgccctcatccgtgcgtttacccgccagagcatgagcacgctcgggaatgcctgggtcgatctgctacgcatcacgttatgggtgctagtccctgtggcgttgttgattgcactgttttttattcaacaaggtgcgctgcaaaactttctgccttatcaggctgtgaataccgttgaaggagcgcaacagctgttacccatggggcctgtagcttctcaggaagcgatcaagatgctcggtactaacggcggtggcttctttaatgccaactcgtcgcatccgtttgaaaacccaaccgcactgaccaacttcgtgcagatgctggcgatcttcttgatcccaacggcgctgtgctttgcctttggtgaagtgatgggcgatcgccgccaggggcgcatgttgctgtgggcgatgtcagtgatttttgtcatctgcgtaggcgtggtgatgtgggcagaagttcagggtaatcctcatctgctggcactgggcacggacagcagcatcaatatggaaggtaaagagagccgtttcggcgtgctggtcagtagcctgtttgcggtcgtgacgacggcggcttcctgtggcgcggtgattgcgatgcatgattcgtttaccgctctcggtggcatggtgccgatgtggctgatgcaaattggtgaagtggtgttcggcggtgtcggttctggtctttacggcatgatgctgtttgtcctgctggcggtgtttattgccgggctgatgattggtcgtacaccggaatatctgggtaaaaaaatcgacgtacgcgagatgaaactgactgcactggcaattctggttaccccgacgctggtgctgatgggcgcggcgttggcgatgatgaccgacgccggacgtagcgccatgctcaaccctggcccgcatggttttagcgaagtgctgtacgccgtgtcatccgccgctaacaacaacggcagcgcctttgccggattaagcgccaactctccgttctggaactgtttactggcgttctgcatgtttgtcggtcgcttcggggtgattatcccggtgatggcaattgccggttcgctggtgagtaaaaagagccaagccgccagctccggcacgctgccaacgcacggcccgctgtttgttggcctgttaatcggcaccgtgttgctggttggcgcactgacctttatccctgccctggcgcttggtccggtggcggaatatctctcctgaatgagtcgtaaacaactggcgctattcgaaccaacacttgtcgttcaggcgctgaaagaagcggtgaaaaaattaaacccgcaggcgcaatggcgcaatccggtgatgtttatcgtctggatcggcagtctgctgaccacctgtattagcatcgcgatggcaagcggtgcgatgcccggcaatgcgctgtttagcgcggccattagcggttggctgtggatcaccgtactgttcgctaatttcgccgaggcgctggcagaaggccgcagtaaagcgcaggccaacagtctgaaaggggtgaaaaaaactgcctttgcccgcaagctgcgtgagccgaaatatggcgctgcggcggacaaagttcctgccgaccaacttcgtaaaggcgatatcgtactggtagaagctggcgatattatcccctgcgatggtgaagttattgaagggggtgcatcggtcgatgaaagcgccatcaccggggaatcggcaccggtgatccgtgaatccggcggcgattttgcctccgtcaccggcggcacgcgtattctttctgactggctggtgattgagtgtagcgttaaccccggcgagacatttctggatcggatgatcgcgatggtggaaggcgcacagcgacgcaaaacgccgaacgaaattgccctgaccattctgctgattgccctgactatcgtctttttactggcaaccgccacgctgtggccgttttccgcgtggggcggtaatgcagtcagcgtaacggtactggtggcgctgctggtctgtctgatcccaaccactattggcggcctgttgtcagcgatcggcgtcgccgggatgagccggatgctaggcgcgaatgtgattgccaccagcggacgtgcagttgaagcggcaggtgacgttgacgttctgctactggataaaaccggcaccatcacactcggtaaccgtcaggcgtcggagtttatccccgcgcagggcgtggatgaaaaaacgctggctgacgccgcacaactggcttcgctggctgatgaaacgccggaaggccgcagtattgtgatcctcgccaagcagcgttttaacctgcgcgagcgcgatgtgcagtcgctccatgccacctttgtaccgtttactgcgcaaagccggatgagcgggatcaacatcgacaaccgcatgatccgtaaaggttctgtcgatgccattcgtcgccatgttgaggctaacggtggtcacttccctaccgatgttgatcaaaaagtcgatcaggttgcgcgtcagggagccacgccgctggtggtggtggaaggttctcgtgtgctgggcgttattgcgctgaaagatatcgtcaaaggcggtattaaagagcgcttcgcccagctgcgcaaaatgggcattaaaacggtgatgattaccggcgataaccgtctgactgccgccgcgattgctgcggaagcgggtgtcgatgattttctcgccgaagcgacaccggaggccaagctggcattgatccgtcagtatcaggcggaaggtcgtttggtagcgatgaccggcgacggcaccaacgatgctccggcgctggcgcaggcagatgtcgcggtggcgatgaactccggcacccaggcggcgaaagaggcgggcaatatggtcgatctcgactctaacccgaccaagttgatcgaggtggtgcacattggcaaacagatgctgatgacccgtggctcgctgaccaccttcagcattgccaacgatgtggcgaaatacttcgccattattccggcggcattcgcggcaacgtatccgcagttaaatgcgctgaacatcatgtgcctgcattcgcccgactccgcaatcctcagtgcggtgattttcaacgccttgattatcgtctttttgattcccctggcgttaaaaggcgtgagttataaaccgcttaccgcttctgccatgttgcgccgtaacttatggatttacggtctgggtgggctgctggtgccgtttatcggtatcaaagtcattgatttactgctgaccgtttgcggtctggtgtgaatgagtggattacgtccggcattatcaacatttatctttctgttattgattactggcggcgtttacccgctgctgaccaccgtactggggcaatggtggtttccctggcaggccaatggttcgttgattcgtgaaggtgatacggtgcgcggttcggcattaatcgggcagaattttaccggcaacggctattttcatggtcgcccgtcggcaacggcagaaatgccctataatccacaggcttctggcgggagcaatctggcggtcagtaaccctgagctggataaactaatagccgcacgcgttgctgcattacgggccgctaacccggatgccagcgcgagcgttccggttgaactggtgacggcatcggcaagcgggctggacaataatatcaccccgcaagcggcggcctggcaaatcccacgcgtggcgaaagcgcgtaatctcagcgttgaacagctcacgcaactgatcgcaaaatacagccaacaaccgctggtgaaatatatcggccagccggttgtcaacattgttgaactcaatctggcgctggataaacttgatgaatatactagagcgcaaaaaaccccgcttcggcggggttttttcgc BBa_B0030_sequence 1 attaaagaggagaaa BBa_K090003_sequence 1 tgagtgcaggcgtgataaccggcgtattgctggtgtttttattactgggttatctggtttatgccctgatcaatgcggaggcgttctgaatggctgcgcaagggttcttactgatcgccacgtttttactggtgttaatggtgctggcgcgtcctttaggcagcgggctggcgcggctgattaatgacattcctcttcccggtacaacgggcgttgagcgcgtactttttcgcgcacttggcgtctctgaccgtgagatgaactggaagcaatatctttgtgccattctcggcctgaacatgctggggctggcggtgctgttttttatgttgctcggtcagcactatctgccgcttaatccacagcagttgccagggctgtcgtgggatctggcgctgaataccgccgtcagctttgtcaccaataccaactggcaatcttatagcggtgaaaccacgttgagctatttcagccagatggcgggcttaacggtgcaaaactttctttctgccgccagcgggattgcggtgatttttgccctcatccgtgcgtttacccgccagagcatgagcacgctcgggaatgcctgggtcgatctgctacgcatcacgttatgggtgctagtccctgtggcgttgttgattgcactgttttttattcaacaaggtgcgctgcaaaactttctgccttatcaggctgtgaataccgttgaaggagcgcaacagctgttacccatggggcctgtagcttctcaggaagcgatcaagatgctcggtactaacggcggtggcttctttaatgccaactcgtcgcatccgtttgaaaacccaaccgcactgaccaacttcgtgcagatgctggcgatcttcttgatcccaacggcgctgtgctttgcctttggtgaagtgatgggcgatcgccgccaggggcgcatgttgctgtgggcgatgtcagtgatttttgtcatctgcgtaggcgtggtgatgtgggcagaagttcagggtaatcctcatctgctggcactgggcacggacagcagcatcaatatggaaggtaaagagagccgtttcggcgtgctggtcagtagcctgtttgcggtcgtgacgacggcggcttcctgtggcgcggtgattgcgatgcatgattcgtttaccgctctcggtggcatggtgccgatgtggctgatgcaaattggtgaagtggtgttcggcggtgtcggttctggtctttacggcatgatgctgtttgtcctgctggcggtgtttattgccgggctgatgattggtcgtacaccggaatatctgggtaaaaaaatcgacgtacgcgagatgaaactgactgcactggcaattctggttaccccgacgctggtgctgatgggcgcggcgttggcgatgatgaccgacgccggacgtagcgccatgctcaaccctggcccgcatggttttagcgaagtgctgtacgccgtgtcatccgccgctaacaacaacggcagcgcctttgccggattaagcgccaactctccgttctggaactgtttactggcgttctgcatgtttgtcggtcgcttcggggtgattatcccggtgatggcaattgccggttcgctggtgagtaaaaagagccaagccgccagctccggcacgctgccaacgcacggcccgctgtttgttggcctgttaatcggcaccgtgttgctggttggcgcactgacctttatccctgccctggcgcttggtccggtggcggaatatctctcctgaatgagtcgtaaacaactggcgctattcgaaccaacacttgtcgttcaggcgctgaaagaagcggtgaaaaaattaaacccgcaggcgcaatggcgcaatccggtgatgtttatcgtctggatcggcagtctgctgaccacctgtattagcatcgcgatggcaagcggtgcgatgcccggcaatgcgctgtttagcgcggccattagcggttggctgtggatcaccgtactgttcgctaatttcgccgaggcgctggcagaaggccgcagtaaagcgcaggccaacagtctgaaaggggtgaaaaaaactgcctttgcccgcaagctgcgtgagccgaaatatggcgctgcggcggacaaagttcctgccgaccaacttcgtaaaggcgatatcgtactggtagaagctggcgatattatcccctgcgatggtgaagttattgaagggggtgcatcggtcgatgaaagcgccatcaccggggaatcggcaccggtgatccgtgaatccggcggcgattttgcctccgtcaccggcggcacgcgtattctttctgactggctggtgattgagtgtagcgttaaccccggcgagacatttctggatcggatgatcgcgatggtggaaggcgcacagcgacgcaaaacgccgaacgaaattgccctgaccattctgctgattgccctgactatcgtctttttactggcaaccgccacgctgtggccgttttccgcgtggggcggtaatgcagtcagcgtaacggtactggtggcgctgctggtctgtctgatcccaaccactattggcggcctgttgtcagcgatcggcgtcgccgggatgagccggatgctaggcgcgaatgtgattgccaccagcggacgtgcagttgaagcggcaggtgacgttgacgttctgctactggataaaaccggcaccatcacactcggtaaccgtcaggcgtcggagtttatccccgcgcagggcgtggatgaaaaaacgctggctgacgccgcacaactggcttcgctggctgatgaaacgccggaaggccgcagtattgtgatcctcgccaagcagcgttttaacctgcgcgagcgcgatgtgcagtcgctccatgccacctttgtaccgtttactgcgcaaagccggatgagcgggatcaacatcgacaaccgcatgatccgtaaaggttctgtcgatgccattcgtcgccatgttgaggctaacggtggtcacttccctaccgatgttgatcaaaaagtcgatcaggttgcgcgtcagggagccacgccgctggtggtggtggaaggttctcgtgtgctgggcgttattgcgctgaaagatatcgtcaaaggcggtattaaagagcgcttcgcccagctgcgcaaaatgggcattaaaacggtgatgattaccggcgataaccgtctgactgccgccgcgattgctgcggaagcgggtgtcgatgattttctcgccgaagcgacaccggaggccaagctggcattgatccgtcagtatcaggcggaaggtcgtttggtagcgatgaccggcgacggcaccaacgatgctccggcgctggcgcaggcagatgtcgcggtggcgatgaactccggcacccaggcggcgaaagaggcgggcaatatggtcgatctcgactctaacccgaccaagttgatcgaggtggtgcacattggcaaacagatgctgatgacccgtggctcgctgaccaccttcagcattgccaacgatgtggcgaaatacttcgccattattccggcggcattcgcggcaacgtatccgcagttaaatgcgctgaacatcatgtgcctgcattcgcccgactccgcaatcctcagtgcggtgattttcaacgccttgattatcgtctttttgattcccctggcgttaaaaggcgtgagttataaaccgcttaccgcttctgccatgttgcgccgtaacttatggatttacggtctgggtgggctgctggtgccgtttatcggtatcaaagtcattgatttactgctgaccgtttgcggtctggtgtgaatgagtggattacgtccggcattatcaacatttatctttctgttattgattactggcggcgtttacccgctgctgaccaccgtactggggcaatggtggtttccctggcaggccaatggttcgttgattcgtgaaggtgatacggtgcgcggttcggcattaatcgggcagaattttaccggcaacggctattttcatggtcgcccgtcggcaacggcagaaatgccctataatccacaggcttctggcgggagcaatctggcggtcagtaaccctgagctggataaactaatagccgcacgcgttgctgcattacgggccgctaacccggatgccagcgcgagcgttccggttgaactggtgacggcatcggcaagcgggctggacaataatatcaccccgcaagcggcggcctggcaaatcccacgcgtggcgaaagcgcgtaatctcagcgttgaacagctcacgcaactgatcgcaaaatacagccaacaaccgctggtgaaatatatcggccagccggttgtcaacattgttgaactcaatctggcgctggataaacttgatgaata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z