BBa_K090402
type: ComponentDefinition
persistent identity: BBa K090402
display id: BBa_K090402
version: 1
was generated by: iGEM to SBOL conversion
description: B. subtilis Episomal Vector with Constitutive GFP
created: 2008-10-29T12:00:00Z
modified: 2015-06-09T09:16:11Z
mutable provenance: See data for BBa_K90401. This part is the same, but with the pUpp promoter, a gram-positive RBS, and a GFP with terminator.
owned by: james
owned by: myers
mutable description: This episomal vector has within its BioBrick region a constitutively expressed GFP gene. It uses the pUpp strong constitutive promoter and a consensus Bacillus RBS sequence.
discontinued: false
dominant: false
group u list: _188_
m user id: 4206
owner id: 3501
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: To build this part, we used 4 different linear DNA pieces assembled with 15 base pair homologous regions PCRed to the ends. These 4 different pieces were then assembled via the InFusion assembly method by Clontech.
star: false
creator: Daniel Goodman
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_E0040
component: BBa_K090505
component: BBa_B0012
component: BBa_B0010
component: BBa_K090504
component: BBa_K090401
sequence annotation: BBa_K090401
sequence annotation: BBa_K090504
sequence annotation: BBa_K090505
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K090402_sequence/1

BBa_E0040
type: Component
persistent identity: component1997010
display id: component1997010
version: 1
top level: BBa_K090402
definition: GFP
access: public

BBa_K090505
type: Component
persistent identity: component1997008
display id: component1997008
version: 1
top level: BBa_K090402
definition: BBa_K090505
access: public

BBa_B0012
type: Component
persistent identity: component1997013
display id: component1997013
version: 1
top level: BBa_K090402
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1997011
display id: component1997011
version: 1
top level: BBa_K090402
definition: BBa_B0010
access: public

BBa_K090504
type: Component
persistent identity: component1997006
display id: component1997006
version: 1
top level: BBa_K090402
definition: BBa_K090504
access: public

BBa_K090401
type: Component
persistent identity: component1997002
display id: component1997002
version: 1
top level: BBa_K090402
definition: pBSEP1
access: public

BBa_K090401
type: SequenceAnnotation
persistent identity: annotation1997002
display id: annotation1997002
version: 1
top level: BBa_K090402
location: https://synbiohub.org/public/igem/BBa_K090402/annotation1997002/range1997002/1
component: BBa_K090401

https://synbiohub.org/public/igem/BBa_K090402/annotation1997002/range1997002/1
type: Range
persistent identity: range1997002
display id: range1997002
version: 1
top level: BBa_K090402
direction: direction/reverse
start: 1
end: 5208
orientation: inline

BBa_K090504
type: SequenceAnnotation
persistent identity: annotation1997006
display id: annotation1997006
version: 1
top level: BBa_K090402
location: https://synbiohub.org/public/igem/BBa_K090402/annotation1997006/range1997006/1
component: BBa_K090504

https://synbiohub.org/public/igem/BBa_K090402/annotation1997006/range1997006/1
type: Range
persistent identity: range1997006
display id: range1997006
version: 1
top level: BBa_K090402
direction: direction/reverse
start: 5217
end: 5455
orientation: inline

BBa_K090505
type: SequenceAnnotation
persistent identity: annotation1997008
display id: annotation1997008
version: 1
top level: BBa_K090402
location: https://synbiohub.org/public/igem/BBa_K090402/annotation1997008/range1997008/1
component: BBa_K090505

https://synbiohub.org/public/igem/BBa_K090402/annotation1997008/range1997008/1
type: Range
persistent identity: range1997008
display id: range1997008
version: 1
top level: BBa_K090402
direction: direction/reverse
start: 5464
end: 5474
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1997010
display id: annotation1997010
version: 1
top level: BBa_K090402
location: https://synbiohub.org/public/igem/BBa_K090402/annotation1997010/range1997010/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K090402/annotation1997010/range1997010/1
type: Range
persistent identity: range1997010
display id: range1997010
version: 1
top level: BBa_K090402
direction: direction/reverse
start: 5481
end: 6200
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1997011
display id: annotation1997011
version: 1
top level: BBa_K090402
location: https://synbiohub.org/public/igem/BBa_K090402/annotation1997011/range1997011/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K090402/annotation1997011/range1997011/1
type: Range
persistent identity: range1997011
display id: range1997011
version: 1
top level: BBa_K090402
direction: direction/forward
start: 6209
end: 6288
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1997013
display id: annotation1997013
version: 1
top level: BBa_K090402
location: https://synbiohub.org/public/igem/BBa_K090402/annotation1997013/range1997013/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K090402/annotation1997013/range1997013/1
type: Range
persistent identity: range1997013
display id: range1997013
version: 1
top level: BBa_K090402
direction: direction/forward
start: 6297
end: 6337
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_K090504
type: ComponentDefinition
persistent identity: BBa K090504
display id: BBa_K090504
version: 1
was generated by: iGEM to SBOL conversion
description: Gram-Positive Strong Constitutive Promoter
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: We PCRed this part out of the pAD43-25 gram positive vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE166.
owned by: james
owned by: myers
mutable description: This is a strong constitutive (always-on) promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli. The promoter comes from the cis-regulatory region of the uracil phosphatase in Bacillus cereus. We functionally annotated the promoter ourselves using the DBTBS database motif search feature. (dbtbs.hgc.jp)
discontinued: false
dominant: false
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: This part generates high expression in gram-positive organisms, and it may be possible to generate deleterious effects on the cell if used to express certain protein products.
star: false
creator: Daniel Goodman
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: putative ComK binding region
sequence annotation: putative SigA -35
sequence annotation: putative SigA -10
sequence: https://synbiohub.org/public/igem/BBa_K090504_sequence/1

putative ComK binding region
type: SequenceAnnotation
persistent identity: annotation1991955
display id: annotation1991955
version: 1
top level: BBa_K090504
location: https://synbiohub.org/public/igem/BBa_K090504/annotation1991955/range1991955/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K090504/annotation1991955/range1991955/1
type: Range
persistent identity: range1991955
display id: range1991955
version: 1
top level: BBa_K090504
direction: direction/forward
start: 76
end: 88
orientation: inline

putative SigA -35
type: SequenceAnnotation
persistent identity: annotation1991956
display id: annotation1991956
version: 1
top level: BBa_K090504
location: https://synbiohub.org/public/igem/BBa_K090504/annotation1991956/range1991956/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K090504/annotation1991956/range1991956/1
type: Range
persistent identity: range1991956
display id: range1991956
version: 1
top level: BBa_K090504
direction: direction/forward
start: 102
end: 108
orientation: inline

putative SigA -10
type: SequenceAnnotation
persistent identity: annotation1991957
display id: annotation1991957
version: 1
top level: BBa_K090504
location: https://synbiohub.org/public/igem/BBa_K090504/annotation1991957/range1991957/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K090504/annotation1991957/range1991957/1
type: Range
persistent identity: range1991957
display id: range1991957
version: 1
top level: BBa_K090504
direction: direction/forward
start: 125
end: 130
orientation: inline

pBSEP1
type: ComponentDefinition
persistent identity: BBa K090401
display id: BBa_K090401
version: 1
was generated by: iGEM to SBOL conversion
description: Gram-positive Shuttle Vector for Episomal Expression
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: This part was made via InFusion assembly from many separate pieces. The backbone comes from PAD123 (Bacillus Stock Center catalog number ECE166), and contains replication origins for B. subtilis and E. coli, as well as resistance markers for Ampicillin and Chloramphenicol.
owned by: james
owned by: myers
mutable description: This episomal plasmid is capable of transformation into both Gram-negative and Gram-positive organisms. It replicates as an episomal plasmid and does not integrate into the genome. For selection in E. coli, use Ampicillin, 100 ??g per mL. For selection in B. subtilis (and other similar gram-positive organisms), use Chloramphenicol, 5 ??g per mL (not 35).
discontinued: false
dominant: false
experience: experience/None
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We had to remove a PstI site inside the coding region of the beta-lactamase gene coding for Ampicillin resistance, and thus the backbone had to be PCRed from two parts, with a joining primer filling in cut site with a degenerate codon.
star: false
creator: Daniel Goodman
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: Biobrick Suffix
sequence annotation: puc9 Plasmid (E. coli rep origin)
sequence annotation: Gram Positive Replication Protein
sequence annotation: Chloramphenicol Acetyl-Transferase
sequence annotation: Beta-Lactamase
sequence annotation: Biobrick Prefix
sequence: https://synbiohub.org/public/igem/BBa_K090401_sequence/1

Biobrick Suffix
type: SequenceAnnotation
persistent identity: annotation1992769
display id: annotation1992769
version: 1
top level: pBSEP1
location: https://synbiohub.org/public/igem/BBa_K090401/annotation1992769/range1992769/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K090401/annotation1992769/range1992769/1
type: Range
persistent identity: range1992769
display id: range1992769
version: 1
top level: pBSEP1
direction: direction/forward
start: 1
end: 21
orientation: inline

puc9 Plasmid (E. coli rep origin)
type: SequenceAnnotation
persistent identity: annotation1992774
display id: annotation1992774
version: 1
top level: pBSEP1
location: https://synbiohub.org/public/igem/BBa_K090401/annotation1992774/range1992774/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K090401/annotation1992774/range1992774/1
type: Range
persistent identity: range1992774
display id: range1992774
version: 1
top level: pBSEP1
direction: direction/forward
start: 399
end: 649
orientation: inline

Gram Positive Replication Protein
type: SequenceAnnotation
persistent identity: annotation1992773
display id: annotation1992773
version: 1
top level: pBSEP1
location: https://synbiohub.org/public/igem/BBa_K090401/annotation1992773/range1992773/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K090401/annotation1992773/range1992773/1
type: Range
persistent identity: range1992773
display id: range1992773
version: 1
top level: pBSEP1
direction: direction/forward
start: 723
end: 1742
orientation: inline

Chloramphenicol Acetyl-Transferase
type: SequenceAnnotation
persistent identity: annotation1992772
display id: annotation1992772
version: 1
top level: pBSEP1
location: https://synbiohub.org/public/igem/BBa_K090401/annotation1992772/range1992772/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K090401/annotation1992772/range1992772/1
type: Range
persistent identity: range1992772
display id: range1992772
version: 1
top level: pBSEP1
direction: direction/forward
start: 2176
end: 2924
orientation: inline

Beta-Lactamase
type: SequenceAnnotation
persistent identity: annotation1992771
display id: annotation1992771
version: 1
top level: pBSEP1
location: https://synbiohub.org/public/igem/BBa_K090401/annotation1992771/range1992771/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K090401/annotation1992771/range1992771/1
type: Range
persistent identity: range1992771
display id: range1992771
version: 1
top level: pBSEP1
direction: direction/reverse
start: 4120
end: 4981
orientation: inline

Biobrick Prefix
type: SequenceAnnotation
persistent identity: annotation1992770
display id: annotation1992770
version: 1
top level: pBSEP1
location: https://synbiohub.org/public/igem/BBa_K090401/annotation1992770/range1992770/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K090401/annotation1992770/range1992770/1
type: Range
persistent identity: range1992770
display id: range1992770
version: 1
top level: pBSEP1
direction: direction/forward
start: 5187
end: 5208
orientation: inline

BBa_K090505
type: ComponentDefinition
persistent identity: BBa K090505
display id: BBa_K090505
version: 1
was generated by: iGEM to SBOL conversion
description: ''Bacillus subtilis'' consensus RBS
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:37Z
mutable provenance: This part was synthesized directly.
owned by: james
owned by: myers
mutable description: This is the consensus RBS for Bacillus subtilis.
discontinued: false
dominant: false
experience: experience/None
group u list: _188_
m user id: 0
owner id: 3501
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The part must be 8 nucleotides away from the first methionine of the coding sequence, and so we added 'TGT' downstream of the actual binding sequence: AAAGGAGG. This, combined with the truncated non-CDS BioBrick prefix scar, gives exactly 8 'spacer bases' between the 3' end of the RBS and the start of the coding sequence.
star: false
creator: Daniel Goodman
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: B. subtilis consensus RBS
sequence: https://synbiohub.org/public/igem/BBa_K090505_sequence/1

B. subtilis consensus RBS
type: SequenceAnnotation
persistent identity: annotation1992802
display id: annotation1992802
version: 1
top level: BBa_K090505
location: https://synbiohub.org/public/igem/BBa_K090505/annotation1992802/range1992802/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K090505/annotation1992802/range1992802/1
type: Range
persistent identity: range1992802
display id: range1992802
version: 1
top level: BBa_K090505
direction: direction/forward
start: 1
end: 8
orientation: inline

https://synbiohub.org/public/igem/BBa_K090402_sequence/1
type: Sequence
persistent identity: BBa K090402 sequence
display id: BBa_K090402_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagaagcttgagtaggacaaatccgccgagcttcgacgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagaacaaagaatacaagaaaatatttacaaaaaatcaatttaacaattccttaaaacatgcaggaattgacgatttaaacaatattagctttgaacaattcttatctcttttcaatagctataaattatttaataagtaagttaagggatgcataaactgcatcccttaacttgtttttcgtgtgcctattttttgtgaatcgctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatacaaaacatatttcaacacaatacaaatgggttagttaaaaaagcaggccttctaaaggtctgctttttttatttgattatgtaatttttaatgccaggatgccaataagccataacctcaaatgcaccatttgcaacctcgtcatcttcttcctcaatcttgaccagatcgccgtcctccgcatcaccaagattcagctctttatgtatctccttcaaaatgccaccgtatccaattaaccttcgagctgccaacgcatcatccaagtaaagcaccgtgttcagattgtcttcagtcaccttattaccgcgcacacaatccgtatccttaaccggatatttagagatttcgcgaacagctttttgctccatcattgcgttccgcacatcgttttcaatctgttcagcgtcaatcttagctttacctttcactcgacgaatatcgacaattggagtgtaatccaatttcatcgcctttttccaaaggctcgtccactccgcctgcttaatatagtttttcccaaaataatttttccttactggtatcaacacatgaaaatgaggatgatatgtatcttcttcatgatttttggtaatctctaaagctctgaaaaatccaagaaccgaagtttttacttttttgtactggaacagtttcctaaagccttccatcatcgcagaaatttgtggcttcagccgttctccctttacatttcgaatcgtcagcgtgagaaaaatccatccgcagccgtactgtctattggcttcctctacgatcaacttattgtgataagcaatttttaacgacctgcgccacgcacacatcggacataacctcactttacaaaaatgggcttgatacagttttaacttgcccgtctccgggtctctcttaaacgaaagatactctgcacaactaattagtttttcagcctttttgccatagtaaggtgccccaatcttactctctaacgcttcgtaatgctccgccatgaggttcgtccgtctctttttccccttccaatcccgctttttacctgttgcggttttatcttcgaggatgctataatcattttcagatgaataaatcaacaaaaaaactccttctgagctagttctctagcattctattattttgattcgacaccttaataatagcagaaggagtttttacctgtcaaagaaccatcaaacccttgatacacaaggctttgacctaattttgaaaaatgatgttgtttctatatagtatcaagataagaaagaaaaggatttttcgctacgctcaaatcctttaaaaaaacacaaaagaccacattttttaatgtggtctttattcttcaactaaagca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K090504_sequence/1
type: Sequence
persistent identity: BBa K090504 sequence
display id: BBa_K090504_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atttttaaagtatgtatacaaatgatgaataaattttggcgatataatgaaggatacagctcccataattggtaaagatactagatagattcatcgtaaaatcatgattttgccaaatttgcccttgaatattagtagcgttttctttacaatcgtaaatagtgtaaaaaagcgtgcaaacgcatgaatatcatctaaaggagagattcacatgggaaaactgtatgtatttgatcctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K090401_sequence/1
type: Sequence
persistent identity: BBa K090401 sequence
display id: BBa_K090401_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagaagcttgagtaggacaaatccgccgagcttcgacgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagaacaaagaatacaagaaaatatttacaaaaaatcaatttaacaattccttaaaacatgcaggaattgacgatttaaacaatattagctttgaacaattcttatctcttttcaatagctataaattatttaataagtaagttaagggatgcataaactgcatcccttaacttgtttttcgtgtgcctattttttgtgaatcgctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatacaaaacatatttcaacacaatacaaatgggttagttaaaaaagcaggccttctaaaggtctgctttttttatttgattatgtaatttttaatgccaggatgccaataagccataacctcaaatgcaccatttgcaacctcgtcatcttcttcctcaatcttgaccagatcgccgtcctccgcatcaccaagattcagctctttatgtatctccttcaaaatgccaccgtatccaattaaccttcgagctgccaacgcatcatccaagtaaagcaccgtgttcagattgtcttcagtcaccttattaccgcgcacacaatccgtatccttaaccggatatttagagatttcgcgaacagctttttgctccatcattgcgttccgcacatcgttttcaatctgttcagcgtcaatcttagctttacctttcactcgacgaatatcgacaattggagtgtaatccaatttcatcgcctttttccaaaggctcgtccactccgcctgcttaatatagtttttcccaaaataatttttccttactggtatcaacacatgaaaatgaggatgatatgtatcttcttcatgatttttggtaatctctaaagctctgaaaaatccaagaaccgaagtttttacttttttgtactggaacagtttcctaaagccttccatcatcgcagaaatttgtggcttcagccgttctccctttacatttcgaatcgtcagcgtgagaaaaatccatccgcagccgtactgtctattggcttcctctacgatcaacttattgtgataagcaatttttaacgacctgcgccacgcacacatcggacataacctcactttacaaaaatgggcttgatacagttttaacttgcccgtctccgggtctctcttaaacgaaagatactctgcacaactaattagtttttcagcctttttgccatagtaaggtgccccaatcttactctctaacgcttcgtaatgctccgccatgaggttcgtccgtctctttttccccttccaatcccgctttttacctgttgcggttttatcttcgaggatgctataatcattttcagatgaataaatcaacaaaaaaactccttctgagctagttctctagcattctattattttgattcgacaccttaataatagcagaaggagtttttacctgtcaaagaaccatcaaacccttgatacacaaggctttgacctaattttgaaaaatgatgttgtttctatatagtatcaagataagaaagaaaaggatttttcgctacgctcaaatcctttaaaaaaacacaaaagaccacattttttaatgtggtctttattcttcaactaaagca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K090505_sequence/1
type: Sequence
persistent identity: BBa K090505 sequence
display id: BBa_K090505_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaggaggtgt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z