LOCUS BBa_K090403 5632 bp DNA linear 25-Nov-2024 DEFINITION Gram-positive Shuttle Vector for Chromosomal Integration ACCESSION BBa_K090403 VERSION BBa_K090403.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=BioBrick prefix CDS 419..1069 /label=chloramphenicol acetyltransferase CDS 1449..2468 /label=amyE back; partial alpha-amylase; similar to Genbank U46196 misc_feature 3070..3689 /label=pBR322 origin CDS 3844..4704 /label=beta-lactamase (ampicillin resistance) promoter 4746..4774 /label=beta-lactamase (ampR) promoter misc_feature 5611..5632 /label=BioBrick suffix ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaattttc 181 gctacgctca aatcctttaa aaaaacacaa aagaccacat tttttaatgt ggtctttatt 241 cttcaactaa agcacccatt agttcaacaa acgaaaattg gataaagtgg gatattttta 301 aaatatatat ttatgttaca gtaatattga cttttaaaaa aggattgatt ctaatgaaga 361 aagcagacaa gtaagcctcc taaattcact ttagataaaa atttaggagg catatcaaat 421 gaactttaat aaaattgatt tagacaattg gaagagaaaa gagatattta atcattattt 481 gaaccaacaa acgactttta gtataaccac agaaattgat attagtgttt tataccgaaa 541 cataaaacaa gaaggatata aattttaccc tgcatttatt ttcttagtga caagggtgat 601 aaactcaaat acagctttta gaactggtta caatagcgac ggagagttag gttattggga 661 taagttagag ccactttata caatttttga tggtgtatct aaaacattct ctggtatttg 721 gactcctgta aagaatgact tcaaagagtt ttatgattta tacctttctg atgtagagaa 781 atataatggt tcggggaaat tgtttcccaa aacacctata cctgaaaatg ctttttctct 841 ttctattatt ccatggactt catttactgg gtttaactta aatatcaata ataatagtaa 901 ttaccttcta cccattatta cagcaggaaa attcattaat aaaggtaatt caatatattt 961 accgctatct ttacaggtac atcattctgt ttgtgatggt tatcatgcag gattgtttat 1021 gaactctatt caggaattgt cagataggcc taatgactgg cttttataat atgagataat 1081 gccgactgta ctttttacag tcggttttct aatgtcacta acctgccccg ttagttgaag 1141 aaggttttta tattacagct ccagatcctc tacgccggac gcatcgtggc cggcatcacc 1201 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg 1261 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg 1321 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc 1381 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt 1441 cgacatggat gagcgatgat gatatccgtt taggctgggc ggtgatagct tctcgttcag 1501 gcagtacgcc tcttttcttt tccagacctg agggaggcgg aaatggtgtg aggttcccgg 1561 ggaaaagcca aataggcgat cgcgggagtg ctttatttga agatcaggct atcactgcgg 1621 tcaatagatt tcacaatgtg atggctggac agcctgagga actctcgaac ccgaatggaa 1681 acaaccagat atttatgaat cagcgcggct cacatggcgt tgtgctggca aatgcaggtt 1741 catcctctgt ctctatcaat acggcaacaa aattgcctga tggcaggtat gacaataaag 1801 ctggagcggg ttcatttcaa gtgaacgatg gtaaactgac aggcacgatc aatgccaggt 1861 ctgtagctgt gctttatcct gatgatattg caaaagcgcc tcatgttttc cttgagaatt 1921 acaaaacagg tgtaacacat tctttcaatg atcaactgac gattaccttg cgtgcagatg 1981 cgaatacaac aaaagccgtt tatcaaatca ataatggacc agacgacagg cgtttaagga 2041 tggagatcaa ttcacaatcg gaaaaggaga tccaatttgg caaaacatac accatcatgt 2101 taaaaggaac gaacagtgat ggtgtaacga ggaccgagaa atacagtttt gttaaaagag 2161 atccagcgtc ggccaaaacc atcggctatc aaaatccgaa tcattggagc caggtaaatg 2221 cttatatcta taaacatgat gggagccgag taattgaatt gaccggatct tggcctggaa 2281 aaccaatgac taaaaatgca gacggaattt acacgctgac gctgcctgcg gacacggata 2341 caaccaacgc aaaagtgatt tttaataatg gcagcgccca agtgcccggt cagaatcagc 2401 ctggctttga ttacgtgcta aatggtttat ataatgactc gggcttaagc ggttctcttc 2461 cccattgagg gcaaggctag acgggactta ccgaaagaaa ccatcaatga tggtttcttt 2521 tttgttcata aatcagacaa aacttttctc ttgcaaaagt ttgtgaagtg ttgcacaata 2581 taaatgtgaa atacttcaca aacaaaaaga catcaaagag aaacataccc tgcaaggatg 2641 ctgatattgt ctgcatttgc gccggagcaa accaaaaacc tggtgagaca cgccttgaat 2701 tagtagaaaa gaacttgaag attttcaaag gcatcgttag tgaagtcatg gcgagcggat 2761 ttgacggcat tttcttagtc gcgacgcgag gctggatggc cttccccatt atgattcttc 2821 tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg 2881 acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta acttcgatca 2941 ctggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg aacgggttgg 3001 catggattgt agggcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 3061 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 3121 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 3181 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 3241 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 3301 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 3361 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 3421 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 3481 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 3541 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 3601 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 3661 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 3721 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 3781 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 3841 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 3901 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 3961 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 4021 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 4081 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 4141 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 4201 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 4261 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 4321 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 4381 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 4441 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 4501 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 4561 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 4621 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 4681 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 4741 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 4801 ggttccgcgc acatttcccc gaaaagtgcc cagtcttcac atcggtttga aaggaggaag 4861 cggaagaatg aagtaagagg gatttttgac tccgaagtaa gtcttcaaaa aatcaaataa 4921 ggagtgtcaa gaatgtttgc aaaacgattc aaaacctctt tactgccgtt attcgctgga 4981 tttttattgc tgtttcattt ggttctggca ggaccggcgg ctgcgagtgc tgaaacggcg 5041 aacaaatcga atgagcttac agcaccgtcg atcaaaagcg gaaccattct tcatgcatgg 5101 aattggtcgt tcaatacgtt aaaacacaat atgaaggata ttcatgatgc aggatataca 5161 gccattcaga catctccgat taaccaagta aaggaaggga atcaaggaga taaaagcatg 5221 tcgaactggt actggctgta tcagccgaca tcgtatcaaa ttggcaaccg ttacttaggt 5281 actgaacaag aatttaaaga aatgtgtgca gccgctgaag aatatggcat aaaggtcatt 5341 gttgacgcgg tcatcaatca taccaccagt gattatgccg cgatttccaa tgaggttaag 5401 agtattccaa actggacaca tggaaacaca caaattaaaa actggtctga tcgatagtac 5461 ataatggatt tccttacgcg aaatacgggc agtgccacct gacgtctaag aaaccattat 5521 tatcatgaca ttaacctata aaaataggcg tatcacgagg cagaatttca gataaaaaaa 5581 atccttagct ttcgctaagg atgatttctg gaattcgcgg ccgcttctag ag //