BBa_K090501
1
BBa_K090501
Gram-Positive IPTG-Inducible Promoter
2008-10-27T12:00:00Z
2015-05-08T01:08:37Z
We PCRed this part out of the pMUTIN-YFP gram positive vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE151.
This is an IPTG-inducible promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli.
false
false
_188_
0
3501
9
It's complicated
false
We found the functional annotation in the associated paper:
Kaltwasser M., T. Wiegert, and W. Schumann. 2002. Construction and Application of Epitope- and Green Fluorescent Protein-Tagging Integration Vectors for Bacillus subtilis. Appl. Environ. Microbiol 68:2624-2628
The primers we used to extract it (sans biobrick ends) are as follow:
Forward: AGAACAACCTCTGCTAAAATTC
Reverse: AAGCTTAATTGTTATCCGCTC
false
Daniel Goodman
annotation1991822
1
-35
range1991822
1
43
48
annotation1991823
1
-10
range1991823
1
66
71
annotation1991824
1
lacOid Operator
range1991824
1
78
97
BBa_K090501_sequence
1
agaacaacctctgctaaaattcctgaaaaattttgcaaaaagttgttgactttatctacaaggtgtggcataatgtgtggaattgtgagcggataacaattaagctt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z