BBa_K090502
1
BBa_K090502
Gram-Positive Xylose-Inducible Promoter
2008-10-27T12:00:00Z
2015-06-09T09:03:37Z
We PCRed this part out of the pSG1154 gram-positive shuttle vector, acquired from the Bacillus Genetic Stock Center (bgsc.org), catalog number ECE153.
This is a Xylose-inducible promoter for use with gram-positive organisms. It requires a downstream gram-positive ribosomal binding site and coding sequence to function. We only have experience using this part in B. subtilis, but it should function in most gram-positive organisms. It has not been tested and will most likely not work in E. coli.
false
false
_188_
4206
3501
9
Not in stock
false
We used the following PCR primers to extract the part, sans biobrick ends:
Forward: TTCATGAAAAACTAAAAAAAATATTG
Reverse: TATGTCATATTGTAAGTAAGTTGCAC
false
Daniel Goodman
annotation1991916
1
-10
range1991916
1
53
58
annotation1991895
1
-35
range1991895
1
30
35
annotation1991917
1
Xylose Repressor Binding Region
range1991917
1
68
91
BBa_K090502_sequence
1
agatctttcatgaaaaactaaaaaaaatattgaaaatactgatgaggttatttaagattaaaataagttagtttgtttgggcaacaaactaatgtgcaacttacttacaatatgacataaaatgca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z