BBa_K090506
1
BBa_K090506
''Bacillus subtilis'' weak RBS
2008-10-27T12:00:00Z
2015-05-08T01:08:37Z
This part was synthesized directly.
This is the weak version of the consensus RBS for Bacillus subtilis. The sequence is present in front of some B. subtilis operons, but is expected to give weaker expression than the consensus sequence.
The consensus sequence AAAGGAGG (BBa_K090505) was changed to AGAGGTGG for this part.
false
false
_188_
0
3501
9
Not in stock
false
The part must be 8 nucleotides away from the first methionine of the coding sequence, and so we added 'TGT' downstream of the actual binding sequence: AGAGGTGG. This, combined with the truncated non-CDS BioBrick prefix scar, gives exactly 8 'spacer bases' between the 3' end of the RBS and the start of the coding sequence.
false
Daniel Goodman
annotation1992815
1
B. subtilis weak RBS
range1992815
1
1
8
BBa_K090506_sequence
1
agaggtggtgt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z