##gff-version 3
##sequence-region BBa_K091134 1 2048
BBa_K091134	.	engineered_region	1008	2048	.	+	0	ID=BBa_S03956;Name=BBa_S03956
BBa_K091134	.	ribosome_entry_site	1173	1185	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S03956
BBa_K091134	.	ribosome_entry_site	1173	1185	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K091134	.	ribosome_entry_site	1179	1182	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K091134	.	engineered_region	1173	1185	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K091134	.	terminator	2008	2048	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03956
BBa_K091134	.	engineered_region	2008	2048	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091134	.	stem_loop	2015	2034	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091134	.	stop_codon	2041	2041	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091134	.	polyA_site	2035	2048	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091134	.	CDS	1192	1911	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_S03956
BBa_K091134	.	CDS	1192	1911	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K091134	.	terminator	1920	1999	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03956
BBa_K091134	.	engineered_region	1920	1999	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091134	.	stem_loop	1931	1974	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091134	.	promoter	1008	1164	.	+	0	ID=BBa_R0079;Name=BBa_R0079;Parent=BBa_S03956
BBa_K091134	.	non_covalent_binding_site	1053	1070	.	+	0	ID=annotation300985;Name=OP2;Parent=BBa_R0079
BBa_K091134	.	non_covalent_binding_site	1113	1132	.	+	0	ID=annotation300992;Name=OP1;Parent=BBa_R0079
BBa_K091134	.	promoter	1147	1152	.	+	0	ID=annotation318496;Name=-10;Parent=BBa_R0079
BBa_K091134	.	promoter	1124	1129	.	+	0	ID=annotation318495;Name=-35;Parent=BBa_R0079
BBa_K091134	.	engineered_region	1	999	.	+	0	ID=BBa_S03966;Name=BBa_S03966
BBa_K091134	.	engineered_region	64	999	.	+	0	ID=BBa_S03954;Name=BBa_S03954;Parent=BBa_S03966
BBa_K091134	.	engineered_region	64	862	.	+	0	ID=BBa_S03156;Name=BBa_S03156;Parent=BBa_S03954
BBa_K091134	.	CDS	82	837	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_S03156
BBa_K091134	.	sequence_alteration	561	561	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K091134	.	CDS	82	798	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K091134	.	engineered_tag	838	862	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K091134	.	tag	799	831	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K091134	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03156
BBa_K091134	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091134	.	terminator	871	999	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S03954
BBa_K091134	.	terminator	959	999	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K091134	.	engineered_region	959	999	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091134	.	stem_loop	966	985	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091134	.	stop_codon	992	992	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091134	.	polyA_site	986	999	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091134	.	terminator	871	950	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K091134	.	engineered_region	871	950	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091134	.	stem_loop	882	925	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091134	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_S03966
BBa_K091134	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K091134	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K091134	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K091134	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K091134	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
>BBa_K091134
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagat
ggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatc
gtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcact
gtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagc
ctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagc
gtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacg
cactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagt
gttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttc
catatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtctta
ttactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggcccctcgctgagcgcgtcc
cggagctgggggcaacctagctgccacctgcttttctgctagctattccagcgaaaacatacagatttccggcgaaatc
aaggctacctgccagttctggcaggtttggccgcgggttctttttggtacacgaaagctactagagtcacacaggaaag
tactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggc
acaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactgg
aaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcat
atgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacg
ggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattt
taaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaa
caaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatc
aacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaa
agatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaa
ctatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatc
tgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata