BBa_K091139 1 BBa_K091139 RBS+lsrR+TT 2008-07-14T11:00:00Z 2015-05-08T01:08:38Z lsrR comes from E. coli MG1655 K091138 with double terminator B0015. false false _191_ 0 3130 9 It's complicated true no false Xiao Zhu component1967015 1 BBa_B0034 component1967016 1 BBa_K091001 component1967017 1 BBa_B0010 component1967019 1 BBa_B0012 annotation1967016 1 BBa_K091001 range1967016 1 19 972 annotation1967017 1 BBa_B0010 range1967017 1 981 1060 annotation1967015 1 BBa_B0034 range1967015 1 1 12 annotation1967019 1 BBa_B0012 range1967019 1 1069 1109 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K091001 1 lsrR LsrR gene 2008-06-18T11:00:00Z 2015-05-08T01:08:37Z The DNA for this part is found in the E. coli strain MG1655. This sequence codes for the LsrR gene which is found in the E. coli strain MG1655. When this protein fors a complex with phosphorylated AI-2 it promotes the LsrA promoter. false false _191_ 0 3080 9 It's complicated true no special considerations false Robert Cool BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K091001_sequence 1 atgacaatcaacgattcggcaatttcagaacagggaatgtgtgaagaagaacaggtcgcgcggatcgcgtggttttactatcacgacgggctgacccagagcgagatcagcgatcgtctcggcctgacacgtttgaaagtgtcgcgattgctggagaaagggcatcagtccggcattattcgcgtacagattaattctcgctttgaaggctgtctggaatatgaaactcaattacgtcgtcagttttcgctgcaacatgtccgggtgatccctgggcttgcggatgctgatgtcggtgggcgactggggataggcgcggcgcatatgttgatgagtttacttcaaccacaacagatgctggcgattggttttggcgaggcaaccatgaatacgctgcaacgcttaagtggttttatttcgtcacagcaaattcgcctggtcacgctctccggtggcgtcggttcttatatgacgggaatcgggcagcttaacgcggcgtgcagtgtgaatattattccggctccgttgcgggcatcctccgctgacattgcccgtacgctaaaaaatgaaaattgcgtcaaagatgttctgttagccgcgcaagcagcggatgtggcgattgtcggcattggtgctgtgagtcaacaggacgatgcgacaatcattcgctccggttatatcagccagggcgaacagttaatgattggccgaaaaggggcggttggcgacattttaggctacttttttgatgcaaaaggtgacgttgtcacgaatatcaaaatacataacgaactgattggcttacctttaagcgcgctgaagaccatacccgtccgggttggcgtggcagggggagaaaataaagccgaagcaattgccgctgcaatgaaaggcggttatatcaacgcactggttaccgatcaggacacagcagcggcgattttacgtagttaa BBa_B0034_sequence 1 aaagaggagaaa BBa_K091139_sequence 1 aaagaggagaaatactagatgacaatcaacgattcggcaatttcagaacagggaatgtgtgaagaagaacaggtcgcgcggatcgcgtggttttactatcacgacgggctgacccagagcgagatcagcgatcgtctcggcctgacacgtttgaaagtgtcgcgattgctggagaaagggcatcagtccggcattattcgcgtacagattaattctcgctttgaaggctgtctggaatatgaaactcaattacgtcgtcagttttcgctgcaacatgtccgggtgatccctgggcttgcggatgctgatgtcggtgggcgactggggataggcgcggcgcatatgttgatgagtttacttcaaccacaacagatgctggcgattggttttggcgaggcaaccatgaatacgctgcaacgcttaagtggttttatttcgtcacagcaaattcgcctggtcacgctctccggtggcgtcggttcttatatgacgggaatcgggcagcttaacgcggcgtgcagtgtgaatattattccggctccgttgcgggcatcctccgctgacattgcccgtacgctaaaaaatgaaaattgcgtcaaagatgttctgttagccgcgcaagcagcggatgtggcgattgtcggcattggtgctgtgagtcaacaggacgatgcgacaatcattcgctccggttatatcagccagggcgaacagttaatgattggccgaaaaggggcggttggcgacattttaggctacttttttgatgcaaaaggtgacgttgtcacgaatatcaaaatacataacgaactgattggcttacctttaagcgcgctgaagaccatacccgtccgggttggcgtggcagggggagaaaataaagccgaagcaattgccgctgcaatgaaaggcggttatatcaacgcactggttaccgatcaggacacagcagcggcgattttacgtagttaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z