##gff-version 3
##sequence-region BBa_K091142 1 1286
BBa_K091142	.	engineered_region	1	810	.	+	0	ID=BBa_K091140;Name=BBa_K091140
BBa_K091142	.	promoter	1	141	.	+	0	ID=BBa_K091106;Name=BBa_K091106;Parent=BBa_K091140
BBa_K091142	.	engineered_region	150	810	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K091140
BBa_K091142	.	CDS	168	785	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K091142	.	engineered_region	168	785	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K091142	.	tag	747	778	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K091142	.	engineered_tag	786	810	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K091142	.	CDS	168	746	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K091142	.	ribosome_entry_site	150	161	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K091142	.	conserved_region	154	157	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091142	.	engineered_region	819	1286	.	+	0	ID=BBa_K091141;Name=BBa_K091141
BBa_K091142	.	engineered_region	819	1149	.	+	0	ID=BBa_I13025;Name=BBa_I13025;Parent=BBa_K091141
BBa_K091142	.	ribosome_entry_site	819	830	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13025
BBa_K091142	.	conserved_region	823	826	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091142	.	CDS	837	1124	.	+	0	ID=BBa_C0072;Name=BBa_C0072;Parent=BBa_I13025
BBa_K091142	.	CDS	837	1085	.	+	0	ID=annotation308499;Name=mnt;Parent=BBa_C0072
BBa_K091142	.	point_mutation	931	933	.	+	0	ID=annotation308502;Name=T;Parent=BBa_C0072
BBa_K091142	.	engineered_tag	1125	1149	.	+	0	ID=annotation2214010;Name=Help:Barcodes;Parent=BBa_C0072
BBa_K091142	.	tag	1086	1118	.	+	0	ID=annotation308503;Name=LVA;Parent=BBa_C0072
BBa_K091142	.	terminator	1158	1286	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K091141
BBa_K091142	.	terminator	1158	1237	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K091142	.	stem_loop	1169	1212	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091142	.	engineered_region	1158	1237	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091142	.	terminator	1246	1286	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K091142	.	engineered_region	1246	1286	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091142	.	stop_codon	1279	1279	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091142	.	stem_loop	1253	1272	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091142	.	polyA_site	1273	1286	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K091142
aaccgtgaaaatcaaaatagcataaattgtgatctattcgtcggaaatatgtgcaatgtccacctaaggttatgaacaa
attaaaagcagaaatacatttaacaccgtgcgtgttgatttatctaacaccgtgcgtgttgatactagagaaagaggag
aaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggtattctaagtc
ttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatgagtatgataa
ctcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacctacaacaggt
gattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatagtcgaattaa
gtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatttgaagctat
atataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgatttttaaagcgt
attaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtattgtctatgc
ctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagcttaataactctg
atagtgctagtgtagatctctactagagaaagaggagaaatactagatggcccgggatgatcctcacttcaattttcgt
atgccaatggaagtaagagagaaattgaaatttagagcagaggcaaacggacggagcatgaactctgagcttttgcaaa
tcgtacaagatgccctaagcaaaccgtcaccagtcactgggtaccgcaatgatgcggaacgactcgccgatgagcagag
cgagttagtgaagaagatggtcttcgatacactgaaggatctttataaaaaaaccaccgctgcaaacgacgaaaactac
gctttagtagcttaataaccctgatagtgctagtgtagatccctactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata