##gff-version 3
##sequence-region BBa_K091144 1 1671
BBa_K091144	.	engineered_region	1	767	.	+	0	ID=BBa_K091132;Name=BBa_K091132
BBa_K091144	.	promoter	1	98	.	+	0	ID=BBa_K091105;Name=BBa_K091105;Parent=BBa_K091132
BBa_K091144	.	operator	1	52	.	+	0	ID=annotation1963742;Name=Mnt;Parent=BBa_K091105
BBa_K091144	.	operator	78	98	.	+	0	ID=annotation1963741;Name=tetR2;Parent=BBa_K091105
BBa_K091144	.	promoter	47	52	.	+	0	ID=annotation1963739;Name=-10;Parent=BBa_K091105
BBa_K091144	.	operator	53	71	.	+	0	ID=annotation1963740;Name=tetR1;Parent=BBa_K091105
BBa_K091144	.	engineered_region	107	767	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K091132
BBa_K091144	.	ribosome_entry_site	107	118	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K091144	.	conserved_region	111	114	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091144	.	CDS	125	742	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K091144	.	CDS	125	703	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K091144	.	tag	704	735	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K091144	.	engineered_tag	743	767	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K091144	.	engineered_region	125	742	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K091144	.	engineered_region	776	1671	.	+	0	ID=BBa_P0455;Name=BBa_P0455
BBa_K091144	.	ribosome_entry_site	776	787	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0455
BBa_K091144	.	conserved_region	780	783	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091144	.	CDS	794	1543	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0455
BBa_K091144	.	tag	1505	1537	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K091144	.	CDS	797	1504	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K091144	.	engineered_tag	1544	1568	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K091144	.	terminator	1577	1617	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0455
BBa_K091144	.	engineered_region	1577	1617	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091144	.	polyA_site	1604	1617	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091144	.	stop_codon	1610	1610	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091144	.	stem_loop	1584	1603	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091144	.	terminator	1626	1671	.	+	0	ID=BBa_B0011;Name=BBa_B0011;Parent=BBa_P0455
BBa_K091144	.	stem_loop	1638	1660	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_K091144	.	engineered_region	1626	1671	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
>BBa_K091144
ctcgaggtgagtgcacagtactaggtccacggtgacctagatctcctatagttccctatcagtgatagagaactgtaat
ccctatcagtgatagagattactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttgg
caattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagt
tgtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaat
gtaagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaac
agagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactc
tgctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaaca
gtaacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatg
tattaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaa
cgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatac
tagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaa
agaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatt
taatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagc
ccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtacc
ctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggt
aagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctcc
aagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagcca
gacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccaca
gtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgttt
ggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagtcac
actggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggct
tttttattattt