LOCUS       BBa_K091148             1997 bp    DNA     linear       15-May-2024
DEFINITION  pLas' Promoter Testing Construct A
ACCESSION   BBa_K091148
VERSION     BBa_K091148.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..778
                     /label=lasR
     misc_feature    779..811
                     /label=LVA
     misc_feature    541..541
                     /label=G
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0079
     misc_feature    44..842
                     /label=BBa_S03156
     stem_loop       862..905
                     /label=stem_loop
     misc_feature    851..930
                     /label=BBa_B0010
     terminator      851..930
                     /label=BBa_B0010
     stem_loop       946..965
                     /label=T7 TE
     misc_feature    972..972
                     /label=stop
     polyA_site      966..979
                     /label=polya
     misc_feature    939..979
                     /label=BBa_B0012
     terminator      939..979
                     /label=BBa_B0012
     terminator      851..979
                     /label=BBa_B0015
     misc_feature    44..979
                     /label=BBa_S03954
     misc_feature    1073..1078
                     /label=-35 Region
     misc_feature    1062..1080
                     /label=LasR Binding Site
     misc_feature    1100..1105
                     /label= -10 Region
     misc_feature    1112..1112
                     /label=Primary Transcriptional Start
     promoter        988..1113
                     /label=BBa_K091117
     RBS             1122..1134
                     /label=RBS-3\Weak
     RBS             1128..1131
                     /label=RBS
     misc_feature    1122..1134
                     /label=BBa_B0032
     RBS             1122..1134
                     /label=BBa_B0032
     CDS             1141..1860
                     /label=GFP protein
     CDS             1141..1860
                     /label=BBa_E0040
     stem_loop       1880..1923
                     /label=stem_loop
     misc_feature    1869..1948
                     /label=BBa_B0010
     terminator      1869..1948
                     /label=BBa_B0010
     stem_loop       1964..1983
                     /label=T7 TE
     misc_feature    1990..1990
                     /label=stop
     polyA_site      1984..1997
                     /label=polya
     misc_feature    1957..1997
                     /label=BBa_B0012
     terminator      1957..1997
                     /label=BBa_B0012
     misc_feature    988..1997
                     /label=BBa_S03981
     misc_feature    44..1997
                     /label=BBa_S03985
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag
      121 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt
      181 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg
      241 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac
      301 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga
      361 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg
      421 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc
      481 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca
      541 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg
      601 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat
      661 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg
      721 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcgc
      781 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc
      841 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      901 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      961 gggcctttct gcgtttatat actagagtgt tctcgtgtga agccattgct ctgatctttt
     1021 ggacgtttct tcgagcctag caagggtccg ggttcaccga aatctatctc atttgctagt
     1081 tataaaatta tgaaatttgt ataaattctt cagtactaga gtcacacagg aaagtactag
     1141 atgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
     1201 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga
     1261 aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
     1321 gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag
     1381 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc
     1441 aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt
     1501 aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa
     1561 ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga
     1621 atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac
     1681 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
     1741 ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt
     1801 cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaataataa
     1861 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1921 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1981 gcctttctgc gtttata
//