##gff-version 3
##sequence-region BBa_K091149 1 1997
BBa_K091149	.	engineered_region	44	1997	.	+	0	ID=BBa_S03986;Name=BBa_S03986
BBa_K091149	.	engineered_region	988	1997	.	+	0	ID=BBa_S03981;Name=BBa_S03981;Parent=BBa_S03986
BBa_K091149	.	promoter	988	1113	.	+	0	ID=BBa_K091117;Name=BBa_K091117;Parent=BBa_S03981
BBa_K091149	.	start_codon	1112	1112	.	+	0	ID=annotation1964075;Name=Primary Transcriptional Start;Parent=BBa_K091117
BBa_K091149	.	sequence_feature	1100	1105	.	+	0	ID=annotation1964074;Name= -10 Region;Parent=BBa_K091117
BBa_K091149	.	sequence_feature	1073	1078	.	+	0	ID=annotation1964072;Name=-35 Region;Parent=BBa_K091117
BBa_K091149	.	non_covalent_binding_site	1062	1080	.	+	0	ID=annotation1964073;Name=LasR Binding Site;Parent=BBa_K091117
BBa_K091149	.	ribosome_entry_site	1122	1134	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S03981
BBa_K091149	.	ribosome_entry_site	1128	1131	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K091149	.	ribosome_entry_site	1122	1134	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K091149	.	engineered_region	1122	1134	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K091149	.	terminator	1957	1997	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03981
BBa_K091149	.	engineered_region	1957	1997	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091149	.	stop_codon	1990	1990	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091149	.	stem_loop	1964	1983	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091149	.	polyA_site	1984	1997	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091149	.	CDS	1141	1860	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_S03981
BBa_K091149	.	CDS	1141	1860	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K091149	.	terminator	1869	1948	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03981
BBa_K091149	.	engineered_region	1869	1948	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091149	.	stem_loop	1880	1923	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091149	.	engineered_region	44	979	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_S03986
BBa_K091149	.	CDS	62	817	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K091149	.	point_mutation	553	553	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K091149	.	CDS	62	811	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K091149	.	engineered_region	62	817	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K091149	.	engineered_tag	818	842	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K091149	.	point_mutation	235	235	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K091149	.	engineered_region	62	63	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K091149	.	terminator	851	930	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K091149	.	engineered_region	851	930	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091149	.	stem_loop	862	905	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091149	.	terminator	939	979	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K091149	.	engineered_region	939	979	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091149	.	stop_codon	972	972	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091149	.	stem_loop	946	965	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091149	.	polyA_site	966	979	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091149	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K091149	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091149	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100
>BBa_K091149
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatgaaaaacataaatgcc
gacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatga
ctaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcct
agataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattct
aactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaag
aagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttt
tgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttct
ctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtt
tagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttcca
tttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaatt
gattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttatatactagagtgttctcgtgtgaagccattgctctgatcttttggacgtt
tcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagttataaaattatgaaatttgtataaat
tcttcagtactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattctt
gttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaac
ttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgt
tcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacag
gaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgtta
atagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactc
acacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagat
ggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccatt
acctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagc
tgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctca
gtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcg
ggtgggcctttctgcgtttata