LOCUS BBa_K091150 2804 bp DNA linear 26-Jun-2024 DEFINITION pLas' Testing Construct C ACCESSION BBa_K091150 VERSION BBa_K091150.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..778 /label=lasR misc_feature 779..811 /label=LVA misc_feature 541..541 /label=G misc_feature 818..842 /label=Help:Barcodes CDS 62..817 /label=BBa_C0079 misc_feature 44..842 /label=BBa_S03156 misc_feature 1..842 /label=BBa_S03982 misc_feature 855..858 /label=conserved RBS 851..862 /label=BBa_B0034 misc_feature 869..870 /label=prefix misc_feature 1042..1042 /label=T misc_feature 1360..1360 /label=A CDS 869..1618 /label=luxR misc_feature 869..1624 /label=BBa_C0062 misc_feature 1625..1649 /label=Help:Barcodes CDS 869..1624 /label=BBa_C0062 stem_loop 1669..1712 /label=stem_loop misc_feature 1658..1737 /label=BBa_B0010 terminator 1658..1737 /label=BBa_B0010 stem_loop 1753..1772 /label=T7 TE misc_feature 1779..1779 /label=stop polyA_site 1773..1786 /label=polya misc_feature 1746..1786 /label=BBa_B0012 terminator 1746..1786 /label=BBa_B0012 misc_feature 851..1786 /label=BBa_I0462 misc_feature 1880..1885 /label=-35 Region misc_feature 1869..1887 /label=LasR Binding Site misc_feature 1907..1912 /label= -10 Region misc_feature 1919..1919 /label=Primary Transcriptional Start promoter 1795..1920 /label=BBa_K091117 RBS 1929..1941 /label=RBS-3\Weak RBS 1935..1938 /label=RBS misc_feature 1929..1941 /label=BBa_B0032 RBS 1929..1941 /label=BBa_B0032 CDS 1948..2667 /label=GFP protein CDS 1948..2667 /label=BBa_E0040 stem_loop 2687..2730 /label=stem_loop misc_feature 2676..2755 /label=BBa_B0010 terminator 2676..2755 /label=BBa_B0010 stem_loop 2771..2790 /label=T7 TE misc_feature 2797..2797 /label=stop polyA_site 2791..2804 /label=polya misc_feature 2764..2804 /label=BBa_B0012 terminator 2764..2804 /label=BBa_B0012 misc_feature 1795..2804 /label=BBa_S03981 misc_feature 851..2804 /label=BBa_S03986 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag 121 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt 181 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg 241 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac 301 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga 361 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg 421 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc 481 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca 541 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg 601 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat 661 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg 721 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcgc 781 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc 841 actactagag aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag 901 aataattaat aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga 961 tatgactaaa atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat 1021 ggttaaatct gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga 1081 tgacgctaat ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc 1141 aattaattgg aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa 1201 agaagcgaaa acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa 1261 tggcttcgga atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt 1321 tttacatgcg tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa 1381 aataaatata gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt 1441 agcgtgggca tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga 1501 gcgtactgtc actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg 1561 ccaaagtatt tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta 1621 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag 1681 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 1741 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtgttct 1801 cgtgtgaagc cattgctctg atcttttgga cgtttcttcg agcctagcaa gggtccgggt 1861 tcaccgaaat ctatctcatt tgctagttat aaaattatga aatttgtata aattcttcag 1921 tactagagtc acacaggaaa gtactagatg cgtaaaggag aagaactttt cactggagtt 1981 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga 2041 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga 2101 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt 2161 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt 2221 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa 2281 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa 2341 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac 2401 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt 2461 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc 2521 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc 2581 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat 2641 ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc 2701 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 2761 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //