LOCUS       BBa_K091150             2804 bp    DNA     linear       26-Jun-2024
DEFINITION  pLas' Testing Construct C
ACCESSION   BBa_K091150
VERSION     BBa_K091150.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..778
                     /label=lasR
     misc_feature    779..811
                     /label=LVA
     misc_feature    541..541
                     /label=G
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0079
     misc_feature    44..842
                     /label=BBa_S03156
     misc_feature    1..842
                     /label=BBa_S03982
     misc_feature    855..858
                     /label=conserved
     RBS             851..862
                     /label=BBa_B0034
     misc_feature    869..870
                     /label=prefix
     misc_feature    1042..1042
                     /label=T
     misc_feature    1360..1360
                     /label=A
     CDS             869..1618
                     /label=luxR
     misc_feature    869..1624
                     /label=BBa_C0062
     misc_feature    1625..1649
                     /label=Help:Barcodes
     CDS             869..1624
                     /label=BBa_C0062
     stem_loop       1669..1712
                     /label=stem_loop
     misc_feature    1658..1737
                     /label=BBa_B0010
     terminator      1658..1737
                     /label=BBa_B0010
     stem_loop       1753..1772
                     /label=T7 TE
     misc_feature    1779..1779
                     /label=stop
     polyA_site      1773..1786
                     /label=polya
     misc_feature    1746..1786
                     /label=BBa_B0012
     terminator      1746..1786
                     /label=BBa_B0012
     misc_feature    851..1786
                     /label=BBa_I0462
     misc_feature    1880..1885
                     /label=-35 Region
     misc_feature    1869..1887
                     /label=LasR Binding Site
     misc_feature    1907..1912
                     /label= -10 Region
     misc_feature    1919..1919
                     /label=Primary Transcriptional Start
     promoter        1795..1920
                     /label=BBa_K091117
     RBS             1929..1941
                     /label=RBS-3\Weak
     RBS             1935..1938
                     /label=RBS
     misc_feature    1929..1941
                     /label=BBa_B0032
     RBS             1929..1941
                     /label=BBa_B0032
     CDS             1948..2667
                     /label=GFP protein
     CDS             1948..2667
                     /label=BBa_E0040
     stem_loop       2687..2730
                     /label=stem_loop
     misc_feature    2676..2755
                     /label=BBa_B0010
     terminator      2676..2755
                     /label=BBa_B0010
     stem_loop       2771..2790
                     /label=T7 TE
     misc_feature    2797..2797
                     /label=stop
     polyA_site      2791..2804
                     /label=polya
     misc_feature    2764..2804
                     /label=BBa_B0012
     terminator      2764..2804
                     /label=BBa_B0012
     misc_feature    1795..2804
                     /label=BBa_S03981
     misc_feature    851..2804
                     /label=BBa_S03986
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag
      121 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt
      181 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg
      241 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac
      301 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga
      361 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg
      421 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc
      481 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca
      541 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg
      601 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat
      661 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg
      721 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcgc
      781 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc
      841 actactagag aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag
      901 aataattaat aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga
      961 tatgactaaa atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat
     1021 ggttaaatct gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga
     1081 tgacgctaat ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc
     1141 aattaattgg aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa
     1201 agaagcgaaa acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa
     1261 tggcttcgga atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt
     1321 tttacatgcg tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa
     1381 aataaatata gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt
     1441 agcgtgggca tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga
     1501 gcgtactgtc actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg
     1561 ccaaagtatt tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta
     1621 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag
     1681 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1741 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtgttct
     1801 cgtgtgaagc cattgctctg atcttttgga cgtttcttcg agcctagcaa gggtccgggt
     1861 tcaccgaaat ctatctcatt tgctagttat aaaattatga aatttgtata aattcttcag
     1921 tactagagtc acacaggaaa gtactagatg cgtaaaggag aagaactttt cactggagtt
     1981 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     2041 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     2101 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt
     2161 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
     2221 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
     2281 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     2341 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     2401 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
     2461 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     2521 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     2581 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     2641 ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc
     2701 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     2761 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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