##gff-version 3
##sequence-region BBa_K091150 1 2804
BBa_K091150	.	engineered_region	1	842	.	+	0	ID=BBa_S03982;Name=BBa_S03982
BBa_K091150	.	promoter	1	35	.	+	0	ID=BBa_J23100;Name=BBa_J23100;Parent=BBa_S03982
BBa_K091150	.	engineered_region	44	842	.	+	0	ID=BBa_S03156;Name=BBa_S03156;Parent=BBa_S03982
BBa_K091150	.	CDS	62	817	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_S03156
BBa_K091150	.	tag	779	811	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K091150	.	sequence_alteration	541	541	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K091150	.	engineered_tag	818	842	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K091150	.	CDS	62	778	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K091150	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03156
BBa_K091150	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091150	.	engineered_region	851	2804	.	+	0	ID=BBa_S03986;Name=BBa_S03986
BBa_K091150	.	engineered_region	1795	2804	.	+	0	ID=BBa_S03981;Name=BBa_S03981;Parent=BBa_S03986
BBa_K091150	.	promoter	1795	1920	.	+	0	ID=BBa_K091117;Name=BBa_K091117;Parent=BBa_S03981
BBa_K091150	.	sequence_feature	1907	1912	.	+	0	ID=annotation1964074;Name= -10 Region;Parent=BBa_K091117
BBa_K091150	.	start_codon	1919	1919	.	+	0	ID=annotation1964075;Name=Primary Transcriptional Start;Parent=BBa_K091117
BBa_K091150	.	sequence_feature	1880	1885	.	+	0	ID=annotation1964072;Name=-35 Region;Parent=BBa_K091117
BBa_K091150	.	non_covalent_binding_site	1869	1887	.	+	0	ID=annotation1964073;Name=LasR Binding Site;Parent=BBa_K091117
BBa_K091150	.	terminator	2764	2804	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03981
BBa_K091150	.	engineered_region	2764	2804	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091150	.	stop_codon	2797	2797	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091150	.	stem_loop	2771	2790	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091150	.	polyA_site	2791	2804	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091150	.	ribosome_entry_site	1929	1941	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S03981
BBa_K091150	.	ribosome_entry_site	1935	1938	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K091150	.	engineered_region	1929	1941	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K091150	.	ribosome_entry_site	1929	1941	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K091150	.	CDS	1948	2667	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_S03981
BBa_K091150	.	CDS	1948	2667	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K091150	.	terminator	2676	2755	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03981
BBa_K091150	.	stem_loop	2687	2730	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091150	.	engineered_region	2676	2755	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091150	.	engineered_region	851	1786	.	+	0	ID=BBa_I0462;Name=BBa_I0462;Parent=BBa_S03986
BBa_K091150	.	terminator	1658	1737	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_K091150	.	stem_loop	1669	1712	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091150	.	engineered_region	1658	1737	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091150	.	ribosome_entry_site	851	862	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_K091150	.	conserved_region	855	858	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091150	.	CDS	869	1624	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_K091150	.	CDS	869	1618	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K091150	.	engineered_region	869	1624	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K091150	.	engineered_tag	1625	1649	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K091150	.	point_mutation	1042	1042	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K091150	.	engineered_region	869	870	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K091150	.	point_mutation	1360	1360	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K091150	.	terminator	1746	1786	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_K091150	.	engineered_region	1746	1786	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091150	.	stop_codon	1779	1779	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091150	.	stem_loop	1753	1772	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091150	.	polyA_site	1773	1786	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K091150
ttgacggctagctcagtcctaggtacagtgctagctactagagaaagaggagaaatactagatggccttggttgacggt
tttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattct
cgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgc
ctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccg
attttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgt
atgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggc
cgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccgga
ctggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcg
gcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcg
gaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgac
gaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactaga
tgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatca
atgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaa
tctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatc
ctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatc
tccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggc
ttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatac
cattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaa
aagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgag
cgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaa
ttttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtgttctcgtgtgaagccattgct
ctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagttataaaatt
atgaaatttgtataaattcttcagtactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactg
gagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtga
tgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcact
actttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgc
ccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttga
aggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattg
gaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaatta
gacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtcct
tttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtcctt
cttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttata