LOCUS       BBa_K091151             2761 bp    DNA     linear       26-Jun-2024
DEFINITION  pLas' Testing Construct D
ACCESSION   BBa_K091151
VERSION     BBa_K091151.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..20
                     /label=prefix
     misc_feature    192..192
                     /label=T
     misc_feature    510..510
                     /label=A
     CDS             19..768
                     /label=luxR
     misc_feature    19..774
                     /label=BBa_C0062
     misc_feature    775..799
                     /label=Help:Barcodes
     CDS             19..774
                     /label=BBa_C0062
     misc_feature    1..799
                     /label=BBa_S01640
     misc_feature    812..815
                     /label=conserved
     RBS             808..819
                     /label=BBa_B0034
     CDS             826..1542
                     /label=lasR
     misc_feature    1543..1575
                     /label=LVA
     misc_feature    1305..1305
                     /label=G
     misc_feature    1582..1606
                     /label=Help:Barcodes
     CDS             826..1581
                     /label=BBa_C0079
     misc_feature    808..1606
                     /label=BBa_S03156
     stem_loop       1626..1669
                     /label=stem_loop
     misc_feature    1615..1694
                     /label=BBa_B0010
     terminator      1615..1694
                     /label=BBa_B0010
     stem_loop       1710..1729
                     /label=T7 TE
     misc_feature    1736..1736
                     /label=stop
     polyA_site      1730..1743
                     /label=polya
     misc_feature    1703..1743
                     /label=BBa_B0012
     terminator      1703..1743
                     /label=BBa_B0012
     terminator      1615..1743
                     /label=BBa_B0015
     misc_feature    808..1743
                     /label=BBa_S03954
     misc_feature    1826..1844
                     /label=LasR
     misc_feature    1845..1864
                     /label=LuxR
     misc_feature    1864..1869
                     /label=-10
     misc_feature    1837..1842
                     /label=-35
     misc_feature    1876..1876
                     /label=Transcriptional Start
     promoter        1752..1877
                     /label=BBa_K091146
     RBS             1886..1898
                     /label=RBS-3\Weak
     RBS             1892..1895
                     /label=RBS
     misc_feature    1886..1898
                     /label=BBa_B0032
     RBS             1886..1898
                     /label=BBa_B0032
     CDS             1905..2624
                     /label=GFP protein
     CDS             1905..2624
                     /label=BBa_E0040
     stem_loop       2644..2687
                     /label=stem_loop
     misc_feature    2633..2712
                     /label=BBa_B0010
     terminator      2633..2712
                     /label=BBa_B0010
     stem_loop       2728..2747
                     /label=T7 TE
     misc_feature    2754..2754
                     /label=stop
     polyA_site      2748..2761
                     /label=polya
     misc_feature    2721..2761
                     /label=BBa_B0012
     terminator      2721..2761
                     /label=BBa_B0012
     misc_feature    1752..2761
                     /label=BBa_S03984
ORIGIN
        1 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat
       61 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa
      121 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct
      181 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat
      241 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg
      301 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa
      361 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga
      421 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg
      481 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata
      541 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca
      601 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc
      661 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt
      721 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga
      781 tagtgctagt gtagatcact actagagaaa gaggagaaat actagatggc cttggttgac
      841 ggttttcttg agctggaacg ctcaagtgga aaattggagt ggagcgccat cctccagaag
      901 atggcgagcg accttggatt ctcgaagatc ctgttcggcc tgttgcctaa ggacagccag
      961 gactacgaga acgccttcat cgtcggcaac tacccggccg cctggcgcga gcattacgac
     1021 cgggctggct acgcgcgggt cgacccgacg gtcagtcact gtacccagag cgtactgccg
     1081 attttctggg aaccgtccat ctaccagacg cgaaagcagc acgagttctt cgaggaagcc
     1141 tcggccgccg gcctggtgta tgggctgacc atgccgctgc atggtgctcg cggcgaactc
     1201 ggcgcgctga gcctcagcgt ggaagcggaa aaccgggccg aggccaaccg tttcatagag
     1261 tcggtcctgc cgaccctgtg gatgctcaag gactacgcac tgcaaagcgg tgccggactg
     1321 gccttcgaac atccggtcag caaaccggtg gttctgacca gccgggagaa ggaagtgttg
     1381 cagtggtgcg ccatcggcaa gaccagttgg gagatatcgg ttatctgcaa ctgctcggaa
     1441 gccaatgtga acttccatat gggaaatatt cggcggaagt tcggtgtgac ctcccgccgc
     1501 gtagcggcca ttatggccgt taatttgggt cttattactc tcgctgcaaa cgacgaaaac
     1561 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc
     1621 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1681 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1741 atatactaga gtgttctcgt gtgaagccat tgctctgatc ttttggacgt ttcttcgagc
     1801 ctagcaaggg tccgggttca ccgaaatcta tctcatttgc tagtacctgt aggatcgtac
     1861 aggtataaat tcttcagtac tagagtcaca caggaaagta ctagatgcgt aaaggagaag
     1921 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
     1981 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
     2041 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt
     2101 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga
     2161 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
     2221 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
     2281 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
     2341 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca
     2401 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
     2461 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
     2521 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
     2581 ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag agccaggcat
     2641 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     2701 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     2761 a
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