##gff-version 3
##sequence-region BBa_K091151 1 2761
BBa_K091151	.	engineered_region	808	1743	.	+	0	ID=BBa_S03954;Name=BBa_S03954
BBa_K091151	.	engineered_region	808	1606	.	+	0	ID=BBa_S03156;Name=BBa_S03156;Parent=BBa_S03954
BBa_K091151	.	CDS	826	1581	.	+	0	ID=BBa_C0079;Name=BBa_C0079;Parent=BBa_S03156
BBa_K091151	.	engineered_tag	1582	1606	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K091151	.	CDS	826	1542	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K091151	.	sequence_alteration	1305	1305	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K091151	.	tag	1543	1575	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K091151	.	ribosome_entry_site	808	819	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03156
BBa_K091151	.	conserved_region	812	815	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091151	.	terminator	1615	1743	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_S03954
BBa_K091151	.	terminator	1615	1694	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K091151	.	engineered_region	1615	1694	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091151	.	stem_loop	1626	1669	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091151	.	terminator	1703	1743	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K091151	.	engineered_region	1703	1743	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091151	.	polyA_site	1730	1743	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091151	.	stop_codon	1736	1736	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091151	.	stem_loop	1710	1729	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091151	.	engineered_region	1	799	.	+	0	ID=BBa_S01640;Name=BBa_S01640
BBa_K091151	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S01640
BBa_K091151	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K091151	.	CDS	19	774	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S01640
BBa_K091151	.	engineered_region	19	20	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K091151	.	point_mutation	510	510	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K091151	.	engineered_region	19	774	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K091151	.	engineered_tag	775	799	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K091151	.	CDS	19	768	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K091151	.	point_mutation	192	192	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K091151	.	engineered_region	1752	2761	.	+	0	ID=BBa_S03984;Name=BBa_S03984
BBa_K091151	.	terminator	2721	2761	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03984
BBa_K091151	.	engineered_region	2721	2761	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K091151	.	polyA_site	2748	2761	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K091151	.	stop_codon	2754	2754	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K091151	.	stem_loop	2728	2747	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K091151	.	CDS	1905	2624	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_S03984
BBa_K091151	.	CDS	1905	2624	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K091151	.	terminator	2633	2712	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03984
BBa_K091151	.	engineered_region	2633	2712	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K091151	.	stem_loop	2644	2687	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K091151	.	ribosome_entry_site	1886	1898	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_S03984
BBa_K091151	.	ribosome_entry_site	1892	1895	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K091151	.	engineered_region	1886	1898	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K091151	.	ribosome_entry_site	1886	1898	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K091151	.	promoter	1752	1877	.	+	0	ID=BBa_K091146;Name=BBa_K091146;Parent=BBa_S03984
BBa_K091151	.	non_covalent_binding_site	1845	1864	.	+	0	ID=annotation1967355;Name=LuxR;Parent=BBa_K091146
BBa_K091151	.	start_codon	1876	1876	.	+	0	ID=annotation1967358;Name=Transcriptional Start;Parent=BBa_K091146
BBa_K091151	.	sequence_feature	1837	1842	.	+	0	ID=annotation1967357;Name=-35;Parent=BBa_K091146
BBa_K091151	.	non_covalent_binding_site	1826	1844	.	+	0	ID=annotation1967354;Name=LasR;Parent=BBa_K091146
BBa_K091151	.	sequence_feature	1864	1869	.	+	0	ID=annotation1967356;Name=-10;Parent=BBa_K091146
>BBa_K091151
aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaa
gcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattta
tcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgct
aatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaaca
atgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctat
tcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttta
catgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaat
caaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaa
aatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgc
caaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagt
gtagatcactactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtgg
aaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcct
aaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctg
gctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctacca
gacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggt
gctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcgg
tcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaa
accggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggtt
atctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcg
tagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaata
acactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttatatactagagtgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggt
tcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagtcacacaggaa
agtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgg
gcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactact
ggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatc
atatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatga
cgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgat
tttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagaca
aacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccatta
tcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcg
aaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatg
aactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtttta
tctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata