LOCUS       BBa_K091152             2804 bp    DNA     linear       15-May-2024
DEFINITION  pLas' Testing Construct D
ACCESSION   BBa_K091152
VERSION     BBa_K091152.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..63
                     /label=prefix
     misc_feature    235..235
                     /label=T
     misc_feature    553..553
                     /label=A
     CDS             62..811
                     /label=luxR
     misc_feature    62..817
                     /label=BBa_C0062
     misc_feature    818..842
                     /label=Help:Barcodes
     CDS             62..817
                     /label=BBa_C0062
     misc_feature    44..842
                     /label=BBa_S01640
     misc_feature    855..858
                     /label=conserved
     RBS             851..862
                     /label=BBa_B0034
     CDS             869..1585
                     /label=lasR
     misc_feature    1586..1618
                     /label=LVA
     misc_feature    1348..1348
                     /label=G
     misc_feature    1625..1649
                     /label=Help:Barcodes
     CDS             869..1624
                     /label=BBa_C0079
     misc_feature    851..1649
                     /label=BBa_S03156
     stem_loop       1669..1712
                     /label=stem_loop
     misc_feature    1658..1737
                     /label=BBa_B0010
     terminator      1658..1737
                     /label=BBa_B0010
     stem_loop       1753..1772
                     /label=T7 TE
     misc_feature    1779..1779
                     /label=stop
     polyA_site      1773..1786
                     /label=polya
     misc_feature    1746..1786
                     /label=BBa_B0012
     terminator      1746..1786
                     /label=BBa_B0012
     terminator      1658..1786
                     /label=BBa_B0015
     misc_feature    851..1786
                     /label=BBa_S03954
     misc_feature    1869..1887
                     /label=LasR
     misc_feature    1888..1907
                     /label=LuxR
     misc_feature    1907..1912
                     /label=-10
     misc_feature    1880..1885
                     /label=-35
     misc_feature    1919..1919
                     /label=Transcriptional Start
     promoter        1795..1920
                     /label=BBa_K091146
     RBS             1929..1941
                     /label=RBS-3\Weak
     RBS             1935..1938
                     /label=RBS
     misc_feature    1929..1941
                     /label=BBa_B0032
     RBS             1929..1941
                     /label=BBa_B0032
     CDS             1948..2667
                     /label=GFP protein
     CDS             1948..2667
                     /label=BBa_E0040
     stem_loop       2687..2730
                     /label=stem_loop
     misc_feature    2676..2755
                     /label=BBa_B0010
     terminator      2676..2755
                     /label=BBa_B0010
     stem_loop       2771..2790
                     /label=T7 TE
     misc_feature    2797..2797
                     /label=stop
     polyA_site      2791..2804
                     /label=polya
     misc_feature    2764..2804
                     /label=BBa_B0012
     terminator      2764..2804
                     /label=BBa_B0012
     misc_feature    1795..2804
                     /label=BBa_S03984
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta aagcttgtag
      121 aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac attgtgaata
      181 ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt caatcctaga
      241 taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa aatatgatcc
      301 tatagtagat tattctaact ccaatcattc accaattaat tggaatatat ttgaaaacaa
      361 tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag gtcttatcac
      421 tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta gttttgcaca
      481 ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga acataccatt
      541 aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata ataaatcaaa
      601 caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag gaaaaagctc
      661 ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc atttaaccaa
      721 tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag caattttaac
      781 aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct agtgtagatc
      841 actactagag aaagaggaga aatactagat ggccttggtt gacggttttc ttgagctgga
      901 acgctcaagt ggaaaattgg agtggagcgc catcctccag aagatggcga gcgaccttgg
      961 attctcgaag atcctgttcg gcctgttgcc taaggacagc caggactacg agaacgcctt
     1021 catcgtcggc aactacccgg ccgcctggcg cgagcattac gaccgggctg gctacgcgcg
     1081 ggtcgacccg acggtcagtc actgtaccca gagcgtactg ccgattttct gggaaccgtc
     1141 catctaccag acgcgaaagc agcacgagtt cttcgaggaa gcctcggccg ccggcctggt
     1201 gtatgggctg accatgccgc tgcatggtgc tcgcggcgaa ctcggcgcgc tgagcctcag
     1261 cgtggaagcg gaaaaccggg ccgaggccaa ccgtttcata gagtcggtcc tgccgaccct
     1321 gtggatgctc aaggactacg cactgcaaag cggtgccgga ctggccttcg aacatccggt
     1381 cagcaaaccg gtggttctga ccagccggga gaaggaagtg ttgcagtggt gcgccatcgg
     1441 caagaccagt tgggagatat cggttatctg caactgctcg gaagccaatg tgaacttcca
     1501 tatgggaaat attcggcgga agttcggtgt gacctcccgc cgcgtagcgg ccattatggc
     1561 cgttaatttg ggtcttatta ctctcgctgc aaacgacgaa aactacgctt tagtagctta
     1621 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag
     1681 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1741 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtgttct
     1801 cgtgtgaagc cattgctctg atcttttgga cgtttcttcg agcctagcaa gggtccgggt
     1861 tcaccgaaat ctatctcatt tgctagtacc tgtaggatcg tacaggtata aattcttcag
     1921 tactagagtc acacaggaaa gtactagatg cgtaaaggag aagaactttt cactggagtt
     1981 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
     2041 gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga
     2101 aaactacctg ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt
     2161 gcgagatacc cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt
     2221 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa
     2281 gtcaagtttg aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa
     2341 gaagatggaa acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac
     2401 atcatggcag acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt
     2461 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     2521 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     2581 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     2641 ggcatggatg aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc
     2701 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     2761 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
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