BBa_K091157
1
BBa_K091157
pLux/Las Hybrid Promoter
2008-07-24T11:00:00Z
2015-05-08T01:08:38Z
The LasR binding site is from the region upstream of the LasI gene in Pseudomonas aeruginosa. The rest of the promoter is the same as BBa_K091156.
K091157 is a modified version of the pLux' promoter (BBa_K091156) containing the addition of a LasR binding site between the -35 and -10 regions. This promoter is expected to be activated in the presence of AI-1 and LuxR and repressed in the presence of PAI-1 and LasR.
false
false
_191_
0
3067
9
Not in stock
true
In order to fit the LasR binding site between the -35 and -10 regions, the last base of the Las Box was cropped and the -35 region was modified to overlap with the first two bases of the Las Box.
false
Erin Feeney
annotation1968423
1
-35 Region
range1968423
1
20
25
annotation1968426
1
LuxR+AI-1
range1968426
1
1
20
annotation1968424
1
-10 Region
range1968424
1
42
47
annotation1968425
1
LasR + PAI-1
range1968425
1
24
42
BBa_K091157_sequence
1
acctgtaggatcgtacaggtttaatctatctcatttgctagtatagtcgaataaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z