LOCUS       BBa_K091161             1103 bp    DNA     linear       22-Dec-2024
DEFINITION  RBS+LasI+RBS+Mnt+TT
ACCESSION   BBa_K091161
VERSION     BBa_K091161.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..627
                     /label=lasI
     CDS             19..627
                     /label=BBa_C0178
     misc_feature    1..627
                     /label=BBa_S03967
     misc_feature    640..643
                     /label=conserved
     RBS             636..647
                     /label=BBa_B0034
     CDS             654..902
                     /label=mnt
     misc_feature    748..750
                     /label=T
     misc_feature    903..935
                     /label=LVA
     misc_feature    942..966
                     /label=Help:Barcodes
     CDS             654..941
                     /label=BBa_C0072
     misc_feature    636..966
                     /label=BBa_I13025
     stem_loop       986..1029
                     /label=stem_loop
     misc_feature    975..1054
                     /label=BBa_B0010
     terminator      975..1054
                     /label=BBa_B0010
     stem_loop       1070..1089
                     /label=T7 TE
     misc_feature    1096..1096
                     /label=stop
     polyA_site      1090..1103
                     /label=polya
     misc_feature    1063..1103
                     /label=BBa_B0012
     terminator      1063..1103
                     /label=BBa_B0012
     terminator      975..1103
                     /label=BBa_B0015
     misc_feature    636..1103
                     /label=BBa_K091141
ORIGIN
        1 aaagaggaga aatactagat gatcgttcag atcggtcgtc gtgaagagtt cgacaaaaaa
       61 ctgctgggtg aaatgcacaa actgcgtgct caggttttca aagaacgtaa aggttgggac
      121 gtttccgtta tcgacgaaat ggaaatcgac ggttacgacg ctctgtcccc gtactacatg
      181 ctgatccagg aagacacccc ggaagctcag gttttcggtt gctggcgtat cttcgacacc
      241 accggtccgt acatgctgaa aaacaccttc ccggaactgc tgcacggtaa agaagctccg
      301 tgctccccgc acatctggga actgtcccgt ttcgctatca actccggtca gaaaggttcc
      361 ctgggtttct ccgactgcac cctggaagct atgcgtgctc tggctcgtta ctccttgcag
      421 aacgacatcc agaccctggt taccgttacc accgttggtg ttgaaaaaat gatgatccgt
      481 gctggtctgg acgtttcccg tttcggtccg cacctgaaaa tcggtatcga acgtgctgtt
      541 gctctgcgta tcgaactgaa cgctaaaacc cagatcgctc tgtacggtgg tgttctggtt
      601 gaacagcgtc tggctgtttc ctaataatac tagagaaaga ggagaaatac tagatggccc
      661 gggatgatcc tcacttcaat tttcgtatgc caatggaagt aagagagaaa ttgaaattta
      721 gagcagaggc aaacggacgg agcatgaact ctgagctttt gcaaatcgta caagatgccc
      781 taagcaaacc gtcaccagtc actgggtacc gcaatgatgc ggaacgactc gccgatgagc
      841 agagcgagtt agtgaagaag atggtcttcg atacactgaa ggatctttat aaaaaaacca
      901 ccgctgcaaa cgacgaaaac tacgctttag tagcttaata accctgatag tgctagtgta
      961 gatccctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1021 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1081 gggtgggcct ttctgcgttt ata
//