LOCUS       BBa_K091162             1168 bp    DNA     linear       22-Dec-2024
DEFINITION  Promoter+RBS+LasI+RBS+Mnt+TT
ACCESSION   BBa_K091162
VERSION     BBa_K091162.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Lux Box
     promoter        20..25
                     /label=Lux -35
     misc_feature    26..43
                     /label=cI OR2
     promoter        44..49
                     /label=Lux -10
     promoter        1..57
                     /label=BBa_K091107
     misc_feature    70..73
                     /label=conserved
     RBS             66..77
                     /label=BBa_B0034
     CDS             84..692
                     /label=lasI
     CDS             84..692
                     /label=BBa_C0178
     misc_feature    66..692
                     /label=BBa_S03967
     misc_feature    705..708
                     /label=conserved
     RBS             701..712
                     /label=BBa_B0034
     CDS             719..967
                     /label=mnt
     misc_feature    813..815
                     /label=T
     misc_feature    968..1000
                     /label=LVA
     misc_feature    1007..1031
                     /label=Help:Barcodes
     CDS             719..1006
                     /label=BBa_C0072
     misc_feature    701..1031
                     /label=BBa_I13025
     stem_loop       1051..1094
                     /label=stem_loop
     misc_feature    1040..1119
                     /label=BBa_B0010
     terminator      1040..1119
                     /label=BBa_B0010
     stem_loop       1135..1154
                     /label=T7 TE
     misc_feature    1161..1161
                     /label=stop
     polyA_site      1155..1168
                     /label=polya
     misc_feature    1128..1168
                     /label=BBa_B0012
     terminator      1128..1168
                     /label=BBa_B0012
     terminator      1040..1168
                     /label=BBa_B0015
     misc_feature    701..1168
                     /label=BBa_K091141
     misc_feature    66..1168
                     /label=BBa_K091161
ORIGIN
        1 acctgtagga tcgtacaggt ttacgtaaca ccgtgcgtgt tgatatagtc gaataaatac
       61 tagagaaaga ggagaaatac tagatgatcg ttcagatcgg tcgtcgtgaa gagttcgaca
      121 aaaaactgct gggtgaaatg cacaaactgc gtgctcaggt tttcaaagaa cgtaaaggtt
      181 gggacgtttc cgttatcgac gaaatggaaa tcgacggtta cgacgctctg tccccgtact
      241 acatgctgat ccaggaagac accccggaag ctcaggtttt cggttgctgg cgtatcttcg
      301 acaccaccgg tccgtacatg ctgaaaaaca ccttcccgga actgctgcac ggtaaagaag
      361 ctccgtgctc cccgcacatc tgggaactgt cccgtttcgc tatcaactcc ggtcagaaag
      421 gttccctggg tttctccgac tgcaccctgg aagctatgcg tgctctggct cgttactcct
      481 tgcagaacga catccagacc ctggttaccg ttaccaccgt tggtgttgaa aaaatgatga
      541 tccgtgctgg tctggacgtt tcccgtttcg gtccgcacct gaaaatcggt atcgaacgtg
      601 ctgttgctct gcgtatcgaa ctgaacgcta aaacccagat cgctctgtac ggtggtgttc
      661 tggttgaaca gcgtctggct gtttcctaat aatactagag aaagaggaga aatactagat
      721 ggcccgggat gatcctcact tcaattttcg tatgccaatg gaagtaagag agaaattgaa
      781 atttagagca gaggcaaacg gacggagcat gaactctgag cttttgcaaa tcgtacaaga
      841 tgccctaagc aaaccgtcac cagtcactgg gtaccgcaat gatgcggaac gactcgccga
      901 tgagcagagc gagttagtga agaagatggt cttcgataca ctgaaggatc tttataaaaa
      961 aaccaccgct gcaaacgacg aaaactacgc tttagtagct taataaccct gatagtgcta
     1021 gtgtagatcc ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1081 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1141 ccttcgggtg ggcctttctg cgtttata
//